Cell Cycle Dynamics of Cultured Coral Endosymbiotic Microalgae (Symbiodinium) Across Different Types (Species) Under Alternate Light and Temperature Conditions

Fujise, L; Nitschke, MR; Frommlet, JC; Serôdio, J; Woodcock, S; Ralph, PJ; Suggett, DJ

Cell Cycle Dynamics of Cultured Coral Endosymbiotic Microalgae (Symbiodinium) Across Different Types (Species) Under Alternate Light and Temperature Conditions

Fujise, L

Nitschke, MR Frommlet, JC Serôdio, J Woodcock, S

Ralph, PJ

Suggett, DJ

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Publication Type:: Journal Article
Citation:: Journal of Eukaryotic Microbiology, 2018, 65 (4), pp. 505 - 517
Issue Date:: 2018-07-01

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Field	Value	Language
dc.contributor.author	Fujise, L https://orcid.org/0000-0002-9423-1365	en_US
dc.contributor.author	Nitschke, MR	en_US
dc.contributor.author	Frommlet, JC	en_US
dc.contributor.author	Serôdio, J	en_US
dc.contributor.author	Woodcock, S https://orcid.org/0000-0003-4903-3164	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Suggett, DJ https://orcid.org/0000-0001-5326-2520	en_US
dc.date.available	2017-12-19	en_US
dc.date.issued	2018-07-01	en_US
dc.identifier.citation	Journal of Eukaryotic Microbiology, 2018, 65 (4), pp. 505 - 517	en_US
dc.identifier.issn	1066-5234	en_US
dc.identifier.uri	http://hdl.handle.net/10453/128392
dc.description.abstract	© 2018 The Author(s) Journal of Eukaryotic Microbiology © 2018 International Society of Protistologists Dinoflagellates of the genus Symbiodinium live in symbiosis with many invertebrates, including reef-building corals. Hosts maintain this symbiosis through continuous regulation of Symbiodinium cell density via expulsion and degradation (postmitotic) and/or constraining cell growth and division through manipulation of the symbiont cell cycle (premitotic). Importance of premitotic regulation is unknown since little data exists on cell cycles for the immense genetic diversity of Symbiodinium. We therefore examined cell cycle progression for several distinct SymbiodiniumITS2-types (B1, C1, D1a). All types exhibited typical microalgal cell cycle progression, G1 phase through to S phase during the light period, and S phase to G2/M phase during the dark period. However, the proportion of cells in these phases differed between strains and reflected differences in growth rates. Undivided larger cells with 3n DNA content were observed especially in type D1a, which exhibited a distinct cell cycle pattern. We further compared cell cycle patterns under different growth light intensities and thermal regimes. Whilst light intensity did not affect cell cycle patterns, heat stress inhibited cell cycle progression and arrested all strains in G1 phase. We discuss the importance of understanding Symbiodinium functional diversity and how our findings apply to clarify stability of host-Symbiodinium symbioses.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP160100271
dc.relation.ispartof	Journal of Eukaryotic Microbiology	en_US
dc.relation.isbasedon	10.1111/jeu.12497	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Anthozoa	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Symbiosis	en_US
dc.subject.mesh	Cell Cycle	en_US
dc.subject.mesh	Light	en_US
dc.subject.mesh	Microalgae	en_US
dc.title	Cell Cycle Dynamics of Cultured Coral Endosymbiotic Microalgae (Symbiodinium) Across Different Types (Species) Under Alternate Light and Temperature Conditions	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	65	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/DVC (Resources)
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	65	en_US

Abstract:

© 2018 The Author(s) Journal of Eukaryotic Microbiology © 2018 International Society of Protistologists Dinoflagellates of the genus Symbiodinium live in symbiosis with many invertebrates, including reef-building corals. Hosts maintain this symbiosis through continuous regulation of Symbiodinium cell density via expulsion and degradation (postmitotic) and/or constraining cell growth and division through manipulation of the symbiont cell cycle (premitotic). Importance of premitotic regulation is unknown since little data exists on cell cycles for the immense genetic diversity of Symbiodinium. We therefore examined cell cycle progression for several distinct SymbiodiniumITS2-types (B1, C1, D1a). All types exhibited typical microalgal cell cycle progression, G1 phase through to S phase during the light period, and S phase to G2/M phase during the dark period. However, the proportion of cells in these phases differed between strains and reflected differences in growth rates. Undivided larger cells with 3n DNA content were observed especially in type D1a, which exhibited a distinct cell cycle pattern. We further compared cell cycle patterns under different growth light intensities and thermal regimes. Whilst light intensity did not affect cell cycle patterns, heat stress inhibited cell cycle progression and arrested all strains in G1 phase. We discuss the importance of understanding Symbiodinium functional diversity and how our findings apply to clarify stability of host-Symbiodinium symbioses.

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http://hdl.handle.net/10453/128392