Quantitative Lateral Flow Strip Sensor Using Highly Doped Upconversion Nanoparticles

He, H; Liu, B; Wen, S; Liao, J; Lin, G; Zhou, J; Jin, D

Quantitative Lateral Flow Strip Sensor Using Highly Doped Upconversion Nanoparticles

He, H

Liu, B Wen, S

Liao, J Lin, G

Zhou, J

Jin, D

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Publication Type:: Journal Article
Citation:: Analytical Chemistry, 2018, 90 (21), pp. 12356 - 12360
Issue Date:: 2018-11-06

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Field	Value	Language
dc.contributor.author	He, H https://orcid.org/0000-0003-3878-3349	en_US
dc.contributor.author	Liu, B	en_US
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658	en_US
dc.contributor.author	Liao, J	en_US
dc.contributor.author	Lin, G https://orcid.org/0000-0001-9880-8478	en_US
dc.contributor.author	Zhou, J https://orcid.org/0000-0002-0605-5745	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.date.issued	2018-11-06	en_US
dc.identifier.citation	Analytical Chemistry, 2018, 90 (21), pp. 12356 - 12360	en_US
dc.identifier.issn	0003-2700	en_US
dc.identifier.uri	http://hdl.handle.net/10453/128507
dc.description.abstract	© 2018 American Chemical Society. Paper-based lateral flow assays, though being low-cost and widely used for rapid in vitro diagnostics, are indicative and do not provide sufficient sensitivity for the detection and quantification of low abundant biomarkers for early stage cancer diagnosis. Here, we design a compact device to create a focused illumination spot with high irradiance, which activates a range of highly doped 50 nm upconversion nanoparticles (UCNPs) to produce orders of magnitude brighter emissions. The device employs a very low-cost laser diode, simplified excitation, and collection optics and permits a mobile phone camera to record the results. Using highly erbium ion (Er 3+ )-doped and thulium ion (Tm 3+ )-doped UCNPs as two independent reporters on two-color lateral flow strips, new records of limit of detection (LOD), 89 and 400 pg/mL, have been achieved for the ultrasensitive detection of prostate specific antigen (PSA) and ephrin type-A receptor 2 (EphA2) biomarkers, respectively, without crosstalk. The technique and device presented in this work suggests a broad scope of low-cost, rapid, and quantitative lateral flow assays in early detection of bioanalytes.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT130100517
dc.relation.ispartof	Analytical Chemistry	en_US
dc.relation.isbasedon	10.1021/acs.analchem.8b04330	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.title	Quantitative Lateral Flow Strip Sensor Using Highly Doped Upconversion Nanoparticles	en_US
dc.type	Journal Article
utslib.citation.volume	21	en_US
utslib.citation.volume	90	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0904 Chemical Engineering	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access
pubs.issue	21	en_US
pubs.publication-status	Published	en_US
pubs.volume	90	en_US

Abstract:

© 2018 American Chemical Society. Paper-based lateral flow assays, though being low-cost and widely used for rapid in vitro diagnostics, are indicative and do not provide sufficient sensitivity for the detection and quantification of low abundant biomarkers for early stage cancer diagnosis. Here, we design a compact device to create a focused illumination spot with high irradiance, which activates a range of highly doped 50 nm upconversion nanoparticles (UCNPs) to produce orders of magnitude brighter emissions. The device employs a very low-cost laser diode, simplified excitation, and collection optics and permits a mobile phone camera to record the results. Using highly erbium ion (Er 3+ )-doped and thulium ion (Tm 3+ )-doped UCNPs as two independent reporters on two-color lateral flow strips, new records of limit of detection (LOD), 89 and 400 pg/mL, have been achieved for the ultrasensitive detection of prostate specific antigen (PSA) and ephrin type-A receptor 2 (EphA2) biomarkers, respectively, without crosstalk. The technique and device presented in this work suggests a broad scope of low-cost, rapid, and quantitative lateral flow assays in early detection of bioanalytes.

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http://hdl.handle.net/10453/128507