Determination of vitamin B<inf>12</inf> in equine urine by liquid chromatography – inductively coupled plasma – mass spectrometry

Wenzel, R; Major, D; Hesp, K; Doble, P

Determination of vitamin B<inf>12</inf> in equine urine by liquid chromatography – inductively coupled plasma – mass spectrometry

Wenzel, R

Major, D Hesp, K Doble, P

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Publication Type:: Journal Article
Citation:: Journal of Trace Elements in Medicine and Biology, 2018, 50 pp. 634 - 639
Issue Date:: 2018-12-01

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Field	Value	Language
dc.contributor.author	Wenzel, R https://orcid.org/0000-0002-4395-0239	en_US
dc.contributor.author	Major, D	en_US
dc.contributor.author	Hesp, K	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.date.available	2018-05-07	en_US
dc.date.issued	2018-12-01	en_US
dc.identifier.citation	Journal of Trace Elements in Medicine and Biology, 2018, 50 pp. 634 - 639	en_US
dc.identifier.issn	0946-672X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/128586
dc.description.abstract	© 2018 Elsevier GmbH Regulating authorities in the racing industry have restricted the administration of potentially performance enhancing cobalt salts to horses. There are severe penalties for trainers presenting horses with elevated urine cobalt concentrations, and compliance is ensured via analysis of total urinary cobalt at thresholds of 100 μg/L. When cobalt is present as part of the cobalamin molecule it is not considered performance enhancing. This paper demonstrates that a horse can excrete a significant proportion of a commercially available vitamin B12 injection in urine without metabolic modification. A liquid chromatography – inductively coupled plasma – mass spectrometry (LC-ICP-MS) method is presented for urinary cobalt speciation. Given the serious nature surrounding performance enhancing drug offences, we conclude that presumptive positives identified by urine total cobalt measurements require further analysis to differentiate inorganic cobalt from vitamin B12.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LP120200079
dc.relation.ispartof	Journal of Trace Elements in Medicine and Biology	en_US
dc.relation.isbasedon	10.1016/j.jtemb.2018.05.005	en_US
dc.subject.classification	Toxicology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Horses	en_US
dc.subject.mesh	Cobalt	en_US
dc.subject.mesh	Vitamin B 12	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.title	Determination of vitamin B<inf>12</inf> in equine urine by liquid chromatography – inductively coupled plasma – mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	50	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	50	en_US

Abstract:

© 2018 Elsevier GmbH Regulating authorities in the racing industry have restricted the administration of potentially performance enhancing cobalt salts to horses. There are severe penalties for trainers presenting horses with elevated urine cobalt concentrations, and compliance is ensured via analysis of total urinary cobalt at thresholds of 100 μg/L. When cobalt is present as part of the cobalamin molecule it is not considered performance enhancing. This paper demonstrates that a horse can excrete a significant proportion of a commercially available vitamin B12 injection in urine without metabolic modification. A liquid chromatography – inductively coupled plasma – mass spectrometry (LC-ICP-MS) method is presented for urinary cobalt speciation. Given the serious nature surrounding performance enhancing drug offences, we conclude that presumptive positives identified by urine total cobalt measurements require further analysis to differentiate inorganic cobalt from vitamin B12.

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http://hdl.handle.net/10453/128586