Roadmaps and Detours: Active Chlorophyll- a Assessments of Primary Productivity Across Marine and Freshwater Systems

Hughes, DJ; Campbell, DA; Doblin, MA; Kromkamp, JC; Lawrenz, E; Moore, CM; Oxborough, K; Prášil, O; Ralph, PJ; Alvarez, MF; Suggett, DJ

Roadmaps and Detours: Active Chlorophyll- a Assessments of Primary Productivity Across Marine and Freshwater Systems

Hughes, DJ

Campbell, DA Doblin, MA

Kromkamp, JC Lawrenz, E Moore, CM Oxborough, K Prášil, O Ralph, PJ

Alvarez, MF Suggett, DJ

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Publication Type:: Journal Article
Citation:: Environmental Science and Technology, 2018, 52 (21), pp. 12039 - 12054
Issue Date:: 2018-11-06

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Field	Value	Language
dc.contributor.author	Hughes, DJ https://orcid.org/0000-0003-3778-7460	en_US
dc.contributor.author	Campbell, DA	en_US
dc.contributor.author	Doblin, MA https://orcid.org/0000-0001-8750-3433	en_US
dc.contributor.author	Kromkamp, JC	en_US
dc.contributor.author	Lawrenz, E	en_US
dc.contributor.author	Moore, CM	en_US
dc.contributor.author	Oxborough, K	en_US
dc.contributor.author	Prášil, O	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Alvarez, MF	en_US
dc.contributor.author	Suggett, DJ https://orcid.org/0000-0001-5326-2520	en_US
dc.date.issued	2018-11-06	en_US
dc.identifier.citation	Environmental Science and Technology, 2018, 52 (21), pp. 12039 - 12054	en_US
dc.identifier.issn	0013-936X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/129581
dc.description.abstract	Copyright © 2018 American Chemical Society. Assessing phytoplankton productivity over space and time remains a core goal for oceanographers and limnologists. Fast Repetition Rate fluorometry (FRRf) provides a potential means to realize this goal with unprecedented resolution and scale yet has not become the "go-to" method despite high expectations. A major obstacle is difficulty converting electron transfer rates to equivalent rates of C-fixation most relevant for studies of biogeochemical C-fluxes. Such difficulty stems from methodological inconsistencies and our limited understanding of how the electron requirement for C-fixation (φ e,C ) is influenced by the environment and by differences in the composition and physiology of phytoplankton assemblages. We outline a "roadmap" for limiting methodological bias and to develop a more mechanistic understanding of the ecophysiology underlying φ e,C . We 1) re-evaluate core physiological processes governing how microalgae invest photosynthetic electron transport-derived energy and reductant into stored carbon versus alternative sinks. Then, we 2) outline steps to facilitate broader uptake and exploitation of FRRf, which could transform our knowledge of aquatic primary productivity. We argue it is time to 3) revise our historic methodological focus on carbon as the currency of choice, to 4) better appreciate that electron transport fundamentally drives ecosystem biogeochemistry, modulates cell-to-cell interactions, and ultimately modifies community biomass and structure.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT130100202
dc.relation.ispartof	Environmental Science and Technology	en_US
dc.relation.isbasedon	10.1021/acs.est.8b03488	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Phytoplankton	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Fresh Water	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Chlorophyll A	en_US
dc.title	Roadmaps and Detours: Active Chlorophyll- a Assessments of Primary Productivity Across Marine and Freshwater Systems	en_US
dc.type	Journal Article
utslib.citation.volume	21	en_US
utslib.citation.volume	52	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0405 Oceanography	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	21	en_US
pubs.publication-status	Published	en_US
pubs.volume	52	en_US

Abstract:

Copyright © 2018 American Chemical Society. Assessing phytoplankton productivity over space and time remains a core goal for oceanographers and limnologists. Fast Repetition Rate fluorometry (FRRf) provides a potential means to realize this goal with unprecedented resolution and scale yet has not become the "go-to" method despite high expectations. A major obstacle is difficulty converting electron transfer rates to equivalent rates of C-fixation most relevant for studies of biogeochemical C-fluxes. Such difficulty stems from methodological inconsistencies and our limited understanding of how the electron requirement for C-fixation (φ e,C ) is influenced by the environment and by differences in the composition and physiology of phytoplankton assemblages. We outline a "roadmap" for limiting methodological bias and to develop a more mechanistic understanding of the ecophysiology underlying φ e,C . We 1) re-evaluate core physiological processes governing how microalgae invest photosynthetic electron transport-derived energy and reductant into stored carbon versus alternative sinks. Then, we 2) outline steps to facilitate broader uptake and exploitation of FRRf, which could transform our knowledge of aquatic primary productivity. We argue it is time to 3) revise our historic methodological focus on carbon as the currency of choice, to 4) better appreciate that electron transport fundamentally drives ecosystem biogeochemistry, modulates cell-to-cell interactions, and ultimately modifies community biomass and structure.

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http://hdl.handle.net/10453/129581