A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Hadzhiev, Y; Qureshi, HK; Wheatley, L; Cooper, L; Jasiulewicz, A; Van Nguyen, H; Wragg, JW; Poovathumkadavil, D; Conic, S; Bajan, S; Sik, A; Hutvàgner, G; Tora, L; Gambus, A; Fossey, JS; Müller, F

A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Hadzhiev, Y

Qureshi, HK Wheatley, L Cooper, L Jasiulewicz, A Van Nguyen, H Wragg, JW Poovathumkadavil, D Conic, S Bajan, S

Sik, A Hutvàgner, G

Tora, L

Gambus, A Fossey, JS

Müller, F

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Publication Type:: Journal Article
Citation:: Nature Communications, 2019, 10 (1)
Issue Date:: 2019-12-01

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Field	Value	Language
dc.contributor.author	Hadzhiev, Y https://orcid.org/0000-0002-1134-4904	en_US
dc.contributor.author	Qureshi, HK	en_US
dc.contributor.author	Wheatley, L	en_US
dc.contributor.author	Cooper, L	en_US
dc.contributor.author	Jasiulewicz, A	en_US
dc.contributor.author	Van Nguyen, H	en_US
dc.contributor.author	Wragg, JW	en_US
dc.contributor.author	Poovathumkadavil, D	en_US
dc.contributor.author	Conic, S	en_US
dc.contributor.author	Bajan, S https://orcid.org/0000-0001-6934-5240	en_US
dc.contributor.author	Sik, A	en_US
dc.contributor.author	Hutvàgner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.contributor.author	Tora, L https://orcid.org/0000-0001-7398-2250	en_US
dc.contributor.author	Gambus, A	en_US
dc.contributor.author	Fossey, JS https://orcid.org/0000-0002-2626-5117	en_US
dc.contributor.author	Müller, F https://orcid.org/0000-0002-0996-774X	en_US
dc.date.available	2019-01-02	en_US
dc.date.issued	2019-12-01	en_US
dc.identifier.citation	Nature Communications, 2019, 10 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130320
dc.description.abstract	© 2019, The Author(s). Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.	en_US
dc.relation.ispartof	Nature Communications	en_US
dc.relation.isbasedon	10.1038/s41467-019-08487-5	en_US
dc.subject.mesh	Zygote	en_US
dc.subject.mesh	Chromosomes	en_US
dc.subject.mesh	Cell Nucleus	en_US
dc.subject.mesh	Chromatin	en_US
dc.subject.mesh	Blastocyst	en_US
dc.subject.mesh	Blastula	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Zebrafish	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Models, Animal	en_US
dc.subject.mesh	Cell Cycle	en_US
dc.subject.mesh	Cell Division	en_US
dc.subject.mesh	S Phase	en_US
dc.subject.mesh	Transcription, Genetic	en_US
dc.subject.mesh	Gene Expression Regulation, Developmental	en_US
dc.subject.mesh	Genome	en_US
dc.subject.mesh	Four-Dimensional Computed Tomography	en_US
dc.subject.mesh	Transcriptome	en_US
dc.subject.mesh	Spatio-Temporal Analysis	en_US
dc.title	A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	10	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

© 2019, The Author(s). Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.

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http://hdl.handle.net/10453/130320