Impact of acute exposure to cigarette smoke on airway gene expression

Billatos, E; Faiz, A; Gesthalter, Y; Leclerc, A; Alekseyev, YO; Xiao, X; Liu, G; Ten Hacken, NHT; Heijink, IH; Timens, W; Brandsma, CA; Postma, DS; van den Berge, M; Spira, A; Lenburg, ME

Impact of acute exposure to cigarette smoke on airway gene expression

Billatos, E Faiz, A

Gesthalter, Y Leclerc, A Alekseyev, YO Xiao, X Liu, G Ten Hacken, NHT Heijink, IH Timens, W Brandsma, CA Postma, DS van den Berge, M Spira, A Lenburg, ME

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Publication Type:: Journal Article
Citation:: Physiological Genomics, 2018, 50 (9), pp. 705 - 713
Issue Date:: 2018-09-01

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Field	Value	Language
dc.contributor.author	Billatos, E	en_US
dc.contributor.author	Faiz, A https://orcid.org/0000-0003-1740-3538	en_US
dc.contributor.author	Gesthalter, Y	en_US
dc.contributor.author	Leclerc, A	en_US
dc.contributor.author	Alekseyev, YO	en_US
dc.contributor.author	Xiao, X	en_US
dc.contributor.author	Liu, G	en_US
dc.contributor.author	Ten Hacken, NHT	en_US
dc.contributor.author	Heijink, IH	en_US
dc.contributor.author	Timens, W	en_US
dc.contributor.author	Brandsma, CA	en_US
dc.contributor.author	Postma, DS	en_US
dc.contributor.author	van den Berge, M	en_US
dc.contributor.author	Spira, A	en_US
dc.contributor.author	Lenburg, ME	en_US
dc.date.issued	2018-09-01	en_US
dc.identifier.citation	Physiological Genomics, 2018, 50 (9), pp. 705 - 713	en_US
dc.identifier.issn	1094-8341	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130587
dc.description.abstract	© 2018 the American Physiological Society. Background: Understanding effects of acute smoke exposure (ASE) on airway epithelial gene expression and their relationship with the effects of chronic smoke exposure may provide biological insights into the development of smoking-related respiratory diseases. Methods: Bronchial airway epithelial cell brushings were collected from 63 individuals without recent cigarette smoke exposure and before and 24 h after smoking three cigarettes. RNA from these samples was profiled on Affymetrix Human Gene 1.0 ST microarrays. Results: We identified 91 genes differentially expressed 24 h after ASE (false discovery rate < 0.25). ASE induced genes involved in xenobiotic metabolism, oxidative stress, and inflammation and repressed genes related to cilium morphogenesis and cell cycle. While many genes altered by ASE are altered similarly in chronic smokers, metallothionein genes are induced by ASE and suppressed in chronic smokers. Metallothioneins are also suppressed in current and former smokers with lung cancer relative to those without lung cancer. Conclusions: Acute exposure to as little as three cigarettes and chronic smoking induce largely concordant changes in airway epithelial gene expression. Differences in short-term and long-term effects of smoking on metallothionein expression and their relationship to lung cancer requires further study given these enzymes’ role in the oxidative stress response.	en_US
dc.relation.ispartof	Physiological Genomics	en_US
dc.relation.isbasedon	10.1152/physiolgenomics.00092.2017	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Bronchi	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Metallothionein	en_US
dc.subject.mesh	Smoking	en_US
dc.subject.mesh	Smoking Cessation	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	Adolescent	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Aged	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Young Adult	en_US
dc.title	Impact of acute exposure to cigarette smoke on airway gene expression	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	50	en_US
utslib.for	1116 Medical Physiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	50	en_US

Abstract:

© 2018 the American Physiological Society. Background: Understanding effects of acute smoke exposure (ASE) on airway epithelial gene expression and their relationship with the effects of chronic smoke exposure may provide biological insights into the development of smoking-related respiratory diseases. Methods: Bronchial airway epithelial cell brushings were collected from 63 individuals without recent cigarette smoke exposure and before and 24 h after smoking three cigarettes. RNA from these samples was profiled on Affymetrix Human Gene 1.0 ST microarrays. Results: We identified 91 genes differentially expressed 24 h after ASE (false discovery rate < 0.25). ASE induced genes involved in xenobiotic metabolism, oxidative stress, and inflammation and repressed genes related to cilium morphogenesis and cell cycle. While many genes altered by ASE are altered similarly in chronic smokers, metallothionein genes are induced by ASE and suppressed in chronic smokers. Metallothioneins are also suppressed in current and former smokers with lung cancer relative to those without lung cancer. Conclusions: Acute exposure to as little as three cigarettes and chronic smoking induce largely concordant changes in airway epithelial gene expression. Differences in short-term and long-term effects of smoking on metallothionein expression and their relationship to lung cancer requires further study given these enzymes’ role in the oxidative stress response.

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http://hdl.handle.net/10453/130587