Length: Vs. stiffness: Which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?

Zhao, J; Lu, H; Yao, Y; Ganda, S; Stenzel, MH

Length: Vs. stiffness: Which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?

Zhao, J Lu, H

Yao, Y Ganda, S Stenzel, MH

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Publication Type:: Journal Article
Citation:: Journal of Materials Chemistry B, 2018, 6 (25), pp. 4223 - 4231
Issue Date:: 2018-01-01

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Field	Value	Language
dc.contributor.author	Zhao, J	en_US
dc.contributor.author	Lu, H https://orcid.org/0000-0001-6932-1900	en_US
dc.contributor.author	Yao, Y	en_US
dc.contributor.author	Ganda, S	en_US
dc.contributor.author	Stenzel, MH https://orcid.org/0000-0002-6433-4419	en_US
dc.date.issued	2018-01-01	en_US
dc.identifier.citation	Journal of Materials Chemistry B, 2018, 6 (25), pp. 4223 - 4231	en_US
dc.identifier.issn	2050-7518	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130637
dc.description.abstract	© 2018 The Royal Society of Chemistry. Polymeric nanoparticles with long circulation time hold great promise for anti-cancer drug delivery. An enhanced circulation effect of rod-like micelles has been reported, yet efficient intracellular delivery, especially their interactions with cells during endocytosis, still remains inconsistent. Internalization of rod-like nanoparticles is significantly affected by a number of factors including aspect ratio, stiffness and surface chemistry of nanoparticles. Our previous research has shown that the length of rods affected their cellular uptake by breast cancer cells. Here, the influence of rod stiffness in cellular uptake was investigated to provide a comprehensive understanding of the interaction between rods and cells during endocytosis. Well-defined fructose-coated rod-like micelles of different lengths and stiffness were prepared successfully. The AFM results indicate that rods based on poly(1-O-MAFru)31-b-PMMA166 are significantly stiffer than those prepared from poly(1-O-MAFru)31-b-PBA158. The cellular uptake of these different rod-like micelles by breast cancer cells was investigated. In vitro studies via 2D and 3D cell culture models reveal that stiffer rods exhibit a higher cellular uptake and a deeper penetration into cells than the soft rod-like micelles. These results indicate that the internalization of rod-like micelles is significantly affected by their stiffness, though the length of rods also plays an important role. Our results yield a fundamental understanding of the stiffness effect of rod-like micelles on cellular uptake.	en_US
dc.relation.ispartof	Journal of Materials Chemistry B	en_US
dc.relation.isbasedon	10.1039/c8tb00706c	en_US
dc.title	Length: Vs. stiffness: Which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?	en_US
dc.type	Journal Article
utslib.citation.volume	25	en_US
utslib.citation.volume	6	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
utslib.for	0903 Biomedical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	25	en_US
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

© 2018 The Royal Society of Chemistry. Polymeric nanoparticles with long circulation time hold great promise for anti-cancer drug delivery. An enhanced circulation effect of rod-like micelles has been reported, yet efficient intracellular delivery, especially their interactions with cells during endocytosis, still remains inconsistent. Internalization of rod-like nanoparticles is significantly affected by a number of factors including aspect ratio, stiffness and surface chemistry of nanoparticles. Our previous research has shown that the length of rods affected their cellular uptake by breast cancer cells. Here, the influence of rod stiffness in cellular uptake was investigated to provide a comprehensive understanding of the interaction between rods and cells during endocytosis. Well-defined fructose-coated rod-like micelles of different lengths and stiffness were prepared successfully. The AFM results indicate that rods based on poly(1-O-MAFru)31-b-PMMA166 are significantly stiffer than those prepared from poly(1-O-MAFru)31-b-PBA158. The cellular uptake of these different rod-like micelles by breast cancer cells was investigated. In vitro studies via 2D and 3D cell culture models reveal that stiffer rods exhibit a higher cellular uptake and a deeper penetration into cells than the soft rod-like micelles. These results indicate that the internalization of rod-like micelles is significantly affected by their stiffness, though the length of rods also plays an important role. Our results yield a fundamental understanding of the stiffness effect of rod-like micelles on cellular uptake.

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http://hdl.handle.net/10453/130637