Multimodal laser ablation/desorption imaging analysis of Zn and MMP-11 in breast tissues

de Vega, RG; Sanchez, MLF; Eiro, N; Vizoso, FJ; Sperling, M; Karst, U; Medel, AS

Multimodal laser ablation/desorption imaging analysis of Zn and MMP-11 in breast tissues

de Vega, RG

Sanchez, MLF Eiro, N Vizoso, FJ Sperling, M Karst, U Medel, AS

Permalink

Publication Type:: Journal Article
Citation:: Analytical and Bioanalytical Chemistry, 2018, 410 (3), pp. 913 - 922
Issue Date:: 2018-01-01

Closed Access

	Filename	Description	Size
	Vega2018_Article_MultimodalLaserAblationDesorpt.pdf	Published Version	1.88 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	de Vega, RG https://orcid.org/0000-0002-9806-9633	en_US
dc.contributor.author	Sanchez, MLF	en_US
dc.contributor.author	Eiro, N	en_US
dc.contributor.author	Vizoso, FJ	en_US
dc.contributor.author	Sperling, M	en_US
dc.contributor.author	Karst, U	en_US
dc.contributor.author	Medel, AS	en_US
dc.date.available	2017-07-19	en_US
dc.date.issued	2018-01-01	en_US
dc.identifier.citation	Analytical and Bioanalytical Chemistry, 2018, 410 (3), pp. 913 - 922	en_US
dc.identifier.issn	1618-2642	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130727
dc.description.abstract	© 2017, Springer-Verlag GmbH Germany. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases. The main functions of these metalloproteinases are the degradation of the stromal connective tissue and basement membrane components. Recent data from model systems suggest that MMPs are involved in breast cancer (BC) initiation, invasion, and metastasis. Particularly, MMP-11 (stromelysin-3) is expressed in stromal fibroblasts adjacent to epithelial tumor cells, and high levels of this metalloproteinase were associated with tumor progression and poor prognosis of BC. Consequently, MMP-11 involved in these processes can be a candidate as a new potential prognostic biomarker in BC. Bioimaging techniques based on laser ablation/desorption and mass spectrometry are rapidly growing in biology and medicine for studies of biological systems to provide information of biomolecules (such as proteins, metabolites, and lipids) and metals with lateral resolution at the micrometer scale. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used for the first time to investigate the distribution of MMP-11 in human breast cancer tissues in order to show a possible correlation between cancerous and healthy samples, by differential proteomics and using such differences for possible cancer diagnosis and/or prognosis. Additionally, those human breast tissue samples were analyzed in parallel by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in order to gather additional information about the elemental distribution of Zn and its possible associations with MMPs.	en_US
dc.relation.ispartof	Analytical and Bioanalytical Chemistry	en_US
dc.relation.isbasedon	10.1007/s00216-017-0537-x	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Breast	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Breast Neoplasms	en_US
dc.subject.mesh	Zinc	en_US
dc.subject.mesh	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Matrix Metalloproteinase 11	en_US
dc.subject.mesh	Optical Imaging	en_US
dc.subject.mesh	Biomarkers, Tumor	en_US
dc.title	Multimodal laser ablation/desorption imaging analysis of Zn and MMP-11 in breast tissues	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	410	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	410	en_US

Abstract:

© 2017, Springer-Verlag GmbH Germany. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases. The main functions of these metalloproteinases are the degradation of the stromal connective tissue and basement membrane components. Recent data from model systems suggest that MMPs are involved in breast cancer (BC) initiation, invasion, and metastasis. Particularly, MMP-11 (stromelysin-3) is expressed in stromal fibroblasts adjacent to epithelial tumor cells, and high levels of this metalloproteinase were associated with tumor progression and poor prognosis of BC. Consequently, MMP-11 involved in these processes can be a candidate as a new potential prognostic biomarker in BC. Bioimaging techniques based on laser ablation/desorption and mass spectrometry are rapidly growing in biology and medicine for studies of biological systems to provide information of biomolecules (such as proteins, metabolites, and lipids) and metals with lateral resolution at the micrometer scale. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used for the first time to investigate the distribution of MMP-11 in human breast cancer tissues in order to show a possible correlation between cancerous and healthy samples, by differential proteomics and using such differences for possible cancer diagnosis and/or prognosis. Additionally, those human breast tissue samples were analyzed in parallel by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in order to gather additional information about the elemental distribution of Zn and its possible associations with MMPs.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/130727