Novel screening test for celiac disease using peptide functionalised gold nanoparticles

Kaur, A; Shimoni, O; Wallach, M

Novel screening test for celiac disease using peptide functionalised gold nanoparticles

Kaur, A

Shimoni, O

Wallach, M

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Publication Type:: Journal Article
Citation:: World Journal of Gastroenterology, 2018, 24 (47), pp. 5379 - 5390
Issue Date:: 2018-12-21

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Field	Value	Language
dc.contributor.author	Kaur, A https://orcid.org/0000-0003-4923-6332	en_US
dc.contributor.author	Shimoni, O https://orcid.org/0000-0001-8822-1024	en_US
dc.contributor.author	Wallach, M	en_US
dc.date.available	2018-10-05	en_US
dc.date.issued	2018-12-21	en_US
dc.identifier.citation	World Journal of Gastroenterology, 2018, 24 (47), pp. 5379 - 5390	en_US
dc.identifier.issn	1007-9327	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130762
dc.description.abstract	© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. AIM To develop a screening test for celiac disease based on the coating of gold nanoparticles with a peptide sequence derived from gliadin, the protein that triggers celiac disease. METHODS 20 nm gold nanoparticles were first coated with NeutrAvidin. A long chain Polyethylene glycol (PEG) linker containing Maleimide at the Ω-end and Biotin group at the α-end was used to ensure peptide coating to the gold nanoparticles. The maleimide group with the thiol (-SH) side chain reacted with the cysteine amino acid in the peptide sequence and the biotinylated and PEGylated peptide was added to the NeutrAvidin coated gold nanoparticles. The peptide coated gold nanoparticles were then converted into a serological assay. We used the peptide functionalised gold nanoparticle-based assay on thirty patient serum samples in a blinded assessment and compared our results with the previously run serological and pathological tests on these patients. RESULTS A stable colloidal suspension of peptide coated gold nanoparticles was obtained without any aggregation. An absorbance peak shift as well as color change was caused by the aggregation of gold nanoparticles following the addition of anti-gliadin antibody to peptide coated nanoparticles at levels associated with celiac disease. The developed assay has been shown to detect anti-gliadin antibody not only in quantitatively spiked samples but also in a small-scale study on real non-hemolytic celiac disease patient’s samples. CONCLUSION The study demonstrates the potential of gold nanoparticle-peptide based approach to be adapted for developing a screening assay for celiac disease diagnosis. The assay could be a part of an exclusion based diagnostic strategy and prove particularly useful for testing high celiac disease risk populations.	en_US
dc.relation	http://purl.org/au-research/grants/arc/IH150100028
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1101258
dc.relation.ispartof	World Journal of Gastroenterology	en_US
dc.relation.isbasedon	10.3748/wjg.v24.i47.5379	en_US
dc.subject.classification	Gastroenterology & Hepatology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Celiac Disease	en_US
dc.subject.mesh	Gold	en_US
dc.subject.mesh	Peptide Fragments	en_US
dc.subject.mesh	Gliadin	en_US
dc.subject.mesh	Autoantibodies	en_US
dc.subject.mesh	Mass Screening	en_US
dc.subject.mesh	Serologic Tests	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Metal Nanoparticles	en_US
dc.title	Novel screening test for celiac disease using peptide functionalised gold nanoparticles	en_US
dc.type	Journal Article
utslib.citation.volume	47	en_US
utslib.citation.volume	24	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.issue	47	en_US
pubs.publication-status	Published	en_US
pubs.volume	24	en_US

Abstract:

© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. AIM To develop a screening test for celiac disease based on the coating of gold nanoparticles with a peptide sequence derived from gliadin, the protein that triggers celiac disease. METHODS 20 nm gold nanoparticles were first coated with NeutrAvidin. A long chain Polyethylene glycol (PEG) linker containing Maleimide at the Ω-end and Biotin group at the α-end was used to ensure peptide coating to the gold nanoparticles. The maleimide group with the thiol (-SH) side chain reacted with the cysteine amino acid in the peptide sequence and the biotinylated and PEGylated peptide was added to the NeutrAvidin coated gold nanoparticles. The peptide coated gold nanoparticles were then converted into a serological assay. We used the peptide functionalised gold nanoparticle-based assay on thirty patient serum samples in a blinded assessment and compared our results with the previously run serological and pathological tests on these patients. RESULTS A stable colloidal suspension of peptide coated gold nanoparticles was obtained without any aggregation. An absorbance peak shift as well as color change was caused by the aggregation of gold nanoparticles following the addition of anti-gliadin antibody to peptide coated nanoparticles at levels associated with celiac disease. The developed assay has been shown to detect anti-gliadin antibody not only in quantitatively spiked samples but also in a small-scale study on real non-hemolytic celiac disease patient’s samples. CONCLUSION The study demonstrates the potential of gold nanoparticle-peptide based approach to be adapted for developing a screening assay for celiac disease diagnosis. The assay could be a part of an exclusion based diagnostic strategy and prove particularly useful for testing high celiac disease risk populations.

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http://hdl.handle.net/10453/130762