In vitro community synergy between bacterial soil isolates can be facilitated by pH stabilization of the environment

Herschend, J; Koren, K; Røder, HL; Brejnrod, A; Kühl, M; Burmølle, M

In vitro community synergy between bacterial soil isolates can be facilitated by pH stabilization of the environment

Herschend, J Koren, K Røder, HL Brejnrod, A Kühl, M

Burmølle, M

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Publication Type:: Journal Article
Citation:: Applied and Environmental Microbiology, 2018, 84 (21)
Issue Date:: 2018-11-01

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Field	Value	Language
dc.contributor.author	Herschend, J	en_US
dc.contributor.author	Koren, K	en_US
dc.contributor.author	Røder, HL	en_US
dc.contributor.author	Brejnrod, A	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Burmølle, M	en_US
dc.date.available	2018-08-19	en_US
dc.date.issued	2018-11-01	en_US
dc.identifier.citation	Applied and Environmental Microbiology, 2018, 84 (21)	en_US
dc.identifier.issn	0099-2240	en_US
dc.identifier.uri	http://hdl.handle.net/10453/131909
dc.description.abstract	© 2018 American Society for Microbiology. The composition and development of naturally occurring microbial communities are defined by a complex interplay between the community and the surrounding environment and by interactions between community members. Intriguingly, these interactions can in some cases cause synergies, where the community is able to outperform its single-species constituents. However, the underlying mechanisms driving community interactions are often unknown and difficult to identify due to high community complexity. Here, we show how opposite pH drift induced by specific community members leads to pH stabilization of the microenvironment, acting as a positive interspecies interaction, driving in vitro community synergy in a model consortium of four coisolated soil bacteria, Microbacterium oxydans, Xanthomonas retroflexus, Stenotrophomonas rhizophila, and Paenibacillus amylolyticus. We use microsensor pH measurements to show how individual species change the local pH microenvironment and how cocultivation leads to a stabilized pH regime over time. Specifically, in vitro acid production from P. amylolyticus and alkali production primarily from X. retroflexus led to an overall pH stabilization of the local environment over time, which in turn resulted in enhanced community growth. This specific type of interspecies interaction was found to be highly dependent on medium type and concentration; however, similar pH drift from the individual species could be observed across medium variants.	en_US
dc.relation.ispartof	Applied and Environmental Microbiology	en_US
dc.relation.isbasedon	10.1128/AEM.01450-18	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Soil	en_US
dc.subject.mesh	Soil Microbiology	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.subject.mesh	Microbiota	en_US
dc.title	In vitro community synergy between bacterial soil isolates can be facilitated by pH stabilization of the environment	en_US
dc.type	Journal Article
utslib.citation.volume	21	en_US
utslib.citation.volume	84	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	MD Multidisciplinary	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	21	en_US
pubs.publication-status	Published	en_US
pubs.volume	84	en_US

Abstract:

© 2018 American Society for Microbiology. The composition and development of naturally occurring microbial communities are defined by a complex interplay between the community and the surrounding environment and by interactions between community members. Intriguingly, these interactions can in some cases cause synergies, where the community is able to outperform its single-species constituents. However, the underlying mechanisms driving community interactions are often unknown and difficult to identify due to high community complexity. Here, we show how opposite pH drift induced by specific community members leads to pH stabilization of the microenvironment, acting as a positive interspecies interaction, driving in vitro community synergy in a model consortium of four coisolated soil bacteria, Microbacterium oxydans, Xanthomonas retroflexus, Stenotrophomonas rhizophila, and Paenibacillus amylolyticus. We use microsensor pH measurements to show how individual species change the local pH microenvironment and how cocultivation leads to a stabilized pH regime over time. Specifically, in vitro acid production from P. amylolyticus and alkali production primarily from X. retroflexus led to an overall pH stabilization of the local environment over time, which in turn resulted in enhanced community growth. This specific type of interspecies interaction was found to be highly dependent on medium type and concentration; however, similar pH drift from the individual species could be observed across medium variants.

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http://hdl.handle.net/10453/131909