Diversity and Distribution in Hypersaline Microbial Mats of Bacteria Related to Chloroflexus spp

Nübel, U; Bateson, MM; Madigan, MT; Kühl, M; Ward, DM

Diversity and Distribution in Hypersaline Microbial Mats of Bacteria Related to Chloroflexus spp

Nübel, U Bateson, MM Madigan, MT Kühl, M

Ward, DM

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Publication Type:: Journal Article
Citation:: Applied and Environmental Microbiology, 2001, 67 (9), pp. 4365 - 4371
Issue Date:: 2001-09-01

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Field	Value	Language
dc.contributor.author	Nübel, U	en_US
dc.contributor.author	Bateson, MM	en_US
dc.contributor.author	Madigan, MT	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Ward, DM	en_US
dc.date.issued	2001-09-01	en_US
dc.identifier.citation	Applied and Environmental Microbiology, 2001, 67 (9), pp. 4365 - 4371	en_US
dc.identifier.issn	0099-2240	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13228
dc.description.abstract	Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern in Guerrero Negro, Mexico, were subjected to cloning or denaturing gradient gel electrophoresis and then sequenced. We found evidence of a high diversity of bacteria related to Chloroflexus which exhibit different distributions along a gradient of salinity from 5.5 to 16%.	en_US
dc.relation.ispartof	Applied and Environmental Microbiology	en_US
dc.relation.isbasedon	10.1128/AEM.67.9.4365-4371.2001	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Chlorobi	en_US
dc.subject.mesh	Sodium Chloride	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	DNA Primers	en_US
dc.subject.mesh	Culture Media	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Water Microbiology	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Genes, rRNA	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Diversity and Distribution in Hypersaline Microbial Mats of Bacteria Related to Chloroflexus spp	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	67	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	67	en_US

Abstract:

Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern in Guerrero Negro, Mexico, were subjected to cloning or denaturing gradient gel electrophoresis and then sequenced. We found evidence of a high diversity of bacteria related to Chloroflexus which exhibit different distributions along a gradient of salinity from 5.5 to 16%.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/13228