φxANES: In vivo imaging of metal-protein coordination environments

James, SA; Hare, DJ; Jenkins, NL; De Jonge, MD; Bush, AI; McColl, G

φxANES: In vivo imaging of metal-protein coordination environments

James, SA Hare, DJ

Jenkins, NL De Jonge, MD Bush, AI McColl, G

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2016, 6
Issue Date:: 2016-02-10

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Field	Value	Language
dc.contributor.author	James, SA	en_US
dc.contributor.author	Hare, DJ https://orcid.org/0000-0002-5922-7643	en_US
dc.contributor.author	Jenkins, NL	en_US
dc.contributor.author	De Jonge, MD	en_US
dc.contributor.author	Bush, AI	en_US
dc.contributor.author	McColl, G	en_US
dc.date.available	2015-12-30	en_US
dc.date.issued	2016-02-10	en_US
dc.identifier.citation	Scientific Reports, 2016, 6	en_US
dc.identifier.uri	http://hdl.handle.net/10453/132282
dc.description.abstract	© 2016, Nature Publishing Group. All rights reserved. We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/srep20350	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Caenorhabditis elegans	en_US
dc.subject.mesh	Iron	en_US
dc.subject.mesh	Organometallic Compounds	en_US
dc.subject.mesh	Metalloproteins	en_US
dc.subject.mesh	Oxidation-Reduction	en_US
dc.subject.mesh	Models, Molecular	en_US
dc.subject.mesh	Image Processing, Computer-Assisted	en_US
dc.subject.mesh	Coordination Complexes	en_US
dc.subject.mesh	X-Ray Absorption Spectroscopy	en_US
dc.title	φxANES: In vivo imaging of metal-protein coordination environments	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.for	0301 Analytical Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

© 2016, Nature Publishing Group. All rights reserved. We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.

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http://hdl.handle.net/10453/132282