Enhanced expression of recombinant proteins in insect cells using a baculovirus vector containing a bacterial leader sequence

Oxford Univ Press United Kingdom
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Journal Article
Nucleic Acids Research, 1992, 20 (22), pp. 6111 - 6112
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Baculovirus/insect cell expression systems have been in use for several years, often resulting in very high levels of_expression of recombinant proteins (1). For expression of mature proteins the preferred vectors, such as pVL941 (2) or p36C (3), utilise the strong polyhedrin promoter and leader sequence. We recently reported the finding that the presence of a 24 bp bacterial sequence immediately upstream of the initiation codon unexpectedly supported high expression levels of tetanus toxin fragment C (4). The bacterial leader sequence is derived from the 3' end of the E.coli trpE gene where it functions as part of the ribosome binding site of the adjacent trpD gene (5). This sequence also enabled P69 pertactin from Bordetella pertussis to be expressed -at high levels by baculovirus~infected cells (unpublished observations). We have investigated this bacterial sequence further. by comparing its effect on the expression of three different heterologous genes.
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