Microparticles released from Mycobacterium tuberculosis-infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15

Hare, NJ; Chan, B; Chan, E; Kaufman, KL; Britton, WJ; Saunders, BM

Microparticles released from Mycobacterium tuberculosis-infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15

Hare, NJ Chan, B Chan, E Kaufman, KL Britton, WJ Saunders, BM

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Publication Type:: Journal Article
Citation:: Proteomics, 2015, 15 (17), pp. 3020 - 3029
Issue Date:: 2015-09-01

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Field	Value	Language
dc.contributor.author	Hare, NJ	en_US
dc.contributor.author	Chan, B	en_US
dc.contributor.author	Chan, E	en_US
dc.contributor.author	Kaufman, KL	en_US
dc.contributor.author	Britton, WJ	en_US
dc.contributor.author	Saunders, BM https://orcid.org/0000-0003-0540-4445	en_US
dc.date.available	2015-05-30	en_US
dc.date.issued	2015-09-01	en_US
dc.identifier.citation	Proteomics, 2015, 15 (17), pp. 3020 - 3029	en_US
dc.identifier.issn	1615-9853	en_US
dc.identifier.uri	http://hdl.handle.net/10453/132541
dc.description.abstract	© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these MPs induced a proinflammatory response from uninfected macrophages in vitro and in vivo following their transfer into uninfected mice. To determine how M. tb infection modulates the protein composition of the MPs, and if this contributes to their proinflammatory properties, we compared the proteomes of MPs derived from M. tb-infected (TBinf-MP) and uninfected human THP-1 monocytic cells. MP proteins were analyzed by GeLC-MS/MS with spectral counting revealing 68 proteins with statistically significant differential abundances. The 42 proteins increased in abundance in TBinf-MPs included proteins associated with immune function (7), lysosomal/endosomal maturation (4), vesicular formation (12), nucleosome proteins (4), and antigen processing (9). Prominent among these were the type I interferon inducible proteins, ISG15, IFIT1, IFIT2, and IFIT3. Exposure of uninfected THP-1 cells to TBinf-MPs induced increased gene expression of isg15, ifit1, ifit2, and ifit3 and the release of proinflammatory cytokines. These proteins may regulate the proinflammatory potential of the MPs and provide candidate biomarkers for M. tb infection.	en_US
dc.relation.ispartof	Proteomics	en_US
dc.relation.isbasedon	10.1002/pmic.201400610	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Monocytes	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mycobacterium tuberculosis	en_US
dc.subject.mesh	Tuberculosis	en_US
dc.subject.mesh	Intracellular Signaling Peptides and Proteins	en_US
dc.subject.mesh	Proteins	en_US
dc.subject.mesh	Carrier Proteins	en_US
dc.subject.mesh	Interferon Type I	en_US
dc.subject.mesh	Ubiquitins	en_US
dc.subject.mesh	Cytokines	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.subject.mesh	Host-Pathogen Interactions	en_US
dc.subject.mesh	Cell-Derived Microparticles	en_US
dc.title	Microparticles released from Mycobacterium tuberculosis-infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15	en_US
dc.type	Journal Article
utslib.citation.volume	17	en_US
utslib.citation.volume	15	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	17	en_US
pubs.publication-status	Published	en_US
pubs.volume	15	en_US

Abstract:

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these MPs induced a proinflammatory response from uninfected macrophages in vitro and in vivo following their transfer into uninfected mice. To determine how M. tb infection modulates the protein composition of the MPs, and if this contributes to their proinflammatory properties, we compared the proteomes of MPs derived from M. tb-infected (TBinf-MP) and uninfected human THP-1 monocytic cells. MP proteins were analyzed by GeLC-MS/MS with spectral counting revealing 68 proteins with statistically significant differential abundances. The 42 proteins increased in abundance in TBinf-MPs included proteins associated with immune function (7), lysosomal/endosomal maturation (4), vesicular formation (12), nucleosome proteins (4), and antigen processing (9). Prominent among these were the type I interferon inducible proteins, ISG15, IFIT1, IFIT2, and IFIT3. Exposure of uninfected THP-1 cells to TBinf-MPs induced increased gene expression of isg15, ifit1, ifit2, and ifit3 and the release of proinflammatory cytokines. These proteins may regulate the proinflammatory potential of the MPs and provide candidate biomarkers for M. tb infection.

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http://hdl.handle.net/10453/132541