A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues
- Publication Type:
- Journal Article
- Parasitology, 1997, 115 (1), pp. 29 - 32
- Issue Date:
A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.
Please use this identifier to cite or link to this item: