A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

Dalton, JP; Day, SR; Drew, AC; Brindley, PJ

A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

Dalton, JP Day, SR Drew, AC Brindley, PJ

Permalink

Publication Type:: Journal Article
Citation:: Parasitology, 1997, 115 (1), pp. 29 - 32
Issue Date:: 1997-08-22

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download full textAdobe PDF (168.94 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Dalton, JP	en_US
dc.contributor.author	Day, SR	en_US
dc.contributor.author	Drew, AC	en_US
dc.contributor.author	Brindley, PJ	en_US
dc.date.issued	1997-08-22	en_US
dc.identifier.citation	Parasitology, 1997, 115 (1), pp. 29 - 32	en_US
dc.identifier.issn	0031-1820	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13264
dc.description.abstract	A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.	en_US
dc.relation.ispartof	Parasitology	en_US
dc.relation.isbasedon	10.1017/S0031182097001091	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Liver	en_US
dc.subject.mesh	Ovum	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Schistosoma japonicum	en_US
dc.subject.mesh	Schistosoma mansoni	en_US
dc.subject.mesh	Schistosomiasis japonica	en_US
dc.subject.mesh	Schistosomiasis mansoni	en_US
dc.subject.mesh	DNA, Helminth	en_US
dc.subject.mesh	DNA, Complementary	en_US
dc.subject.mesh	Blotting, Southern	en_US
dc.subject.mesh	Parasitology	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	115	en_US
utslib.for	060502 Infectious Agents	en_US
utslib.for	060107 Enzymes	en_US
utslib.for	110803 Medical Parasitology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	115	en_US

Abstract:

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/13264