Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France)

Fourçans, A; De Oteyza, TG; Wieland, A; Solé, A; Diestra, E; Van Bleijswijk, J; Grimalt, JO; Kühl, M; Esteve, I; Muyzer, G; Caumette, P; Duran, R

Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France)

Fourçans, A De Oteyza, TG Wieland, A Solé, A Diestra, E Van Bleijswijk, J Grimalt, JO Kühl, M

Esteve, I Muyzer, G Caumette, P Duran, R

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Publication Type:: Journal Article
Citation:: FEMS Microbiology Ecology, 2004, 51 (1), pp. 55 - 70
Issue Date:: 2004-12-27

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Field	Value	Language
dc.contributor.author	Fourçans, A	en_US
dc.contributor.author	De Oteyza, TG	en_US
dc.contributor.author	Wieland, A	en_US
dc.contributor.author	Solé, A	en_US
dc.contributor.author	Diestra, E	en_US
dc.contributor.author	Van Bleijswijk, J	en_US
dc.contributor.author	Grimalt, JO	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Esteve, I	en_US
dc.contributor.author	Muyzer, G	en_US
dc.contributor.author	Caumette, P	en_US
dc.contributor.author	Duran, R	en_US
dc.date.available	2004-07-13	en_US
dc.date.issued	2004-12-27	en_US
dc.identifier.citation	FEMS Microbiology Ecology, 2004, 51 (1), pp. 55 - 70	en_US
dc.identifier.issn	0168-6496	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13294
dc.description.abstract	A photosynthetic microbial mat was investigated in a large pond of a Mediterranean saltern (Salins-de-Giraud, Camargue, France) having water salinity from 70‰ to 150‰ (w/v). Analysis of characteristic biomarkers (e.g., major microbial fatty acids, hydrocarbons, alcohols and alkenones) revealed that cyanobacteria were the major component of the pond, in addition to diatoms and other algae. Functional bacterial groups involved in the sulfur cycle could be correlated to these biomarkers, i.e. sulfate-reducing, sulfur-oxidizing and anoxygenic phototrophic bacteria. In the first 0.5 mm of the mat, a high rate of photosynthesis showed the activity of oxygenic phototrophs in the surface layer. Ten different cyanobacterial populations were detected with confocal laser scanning microscopy: six filamentous species, with Microcoleus chthonoplastes and Halomicronema excentricum as dominant (73% of total counts); and four unicellular types affiliated to Microcystis, Chroococcus, Gloeocapsa, and Synechocystis (27% of total counts). Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments confirmed the presence of Microcoleus, Oscillatoria, and Leptolyngbya strains (Halomicronema was not detected here) and revealed additional presence of Phormidium, Pleurocapsa and Calotrix types. Spectral scalar irradiance measurements did not reveal a particular zonation of cyanobacteria, purple or green bacteria in the first millimeter of the mat. Terminal-restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA gene fragments of bacteria depicted the community composition and a fine-scale depth-distribution of at least five different populations of anoxygenic phototrophs and at least three types of sulfate-reducing bacteria along the microgradients of oxygen and light inside the microbial mat. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	FEMS Microbiology Ecology	en_US
dc.relation.isbasedon	10.1016/j.femsec.2004.07.012	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Cyanobacteria	en_US
dc.subject.mesh	Sodium Chloride	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Biological Markers	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Population Dynamics	en_US
dc.subject.mesh	Water Supply	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Geology	en_US
dc.subject.mesh	France	en_US
dc.subject.mesh	Geological Phenomena	en_US
dc.subject.mesh	Biomarkers	en_US
dc.title	Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France)	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	51	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	51	en_US

Abstract:

A photosynthetic microbial mat was investigated in a large pond of a Mediterranean saltern (Salins-de-Giraud, Camargue, France) having water salinity from 70‰ to 150‰ (w/v). Analysis of characteristic biomarkers (e.g., major microbial fatty acids, hydrocarbons, alcohols and alkenones) revealed that cyanobacteria were the major component of the pond, in addition to diatoms and other algae. Functional bacterial groups involved in the sulfur cycle could be correlated to these biomarkers, i.e. sulfate-reducing, sulfur-oxidizing and anoxygenic phototrophic bacteria. In the first 0.5 mm of the mat, a high rate of photosynthesis showed the activity of oxygenic phototrophs in the surface layer. Ten different cyanobacterial populations were detected with confocal laser scanning microscopy: six filamentous species, with Microcoleus chthonoplastes and Halomicronema excentricum as dominant (73% of total counts); and four unicellular types affiliated to Microcystis, Chroococcus, Gloeocapsa, and Synechocystis (27% of total counts). Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments confirmed the presence of Microcoleus, Oscillatoria, and Leptolyngbya strains (Halomicronema was not detected here) and revealed additional presence of Phormidium, Pleurocapsa and Calotrix types. Spectral scalar irradiance measurements did not reveal a particular zonation of cyanobacteria, purple or green bacteria in the first millimeter of the mat. Terminal-restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA gene fragments of bacteria depicted the community composition and a fine-scale depth-distribution of at least five different populations of anoxygenic phototrophs and at least three types of sulfate-reducing bacteria along the microgradients of oxygen and light inside the microbial mat. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/13294