The Role Of A Stress-Response Protein In Bacterial Virulence

Editions Scientifiques Elsevier
Publication Type:
Journal Article
Research in Microbiology, 1990, 141 (7-8), pp. 823 - 825
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We have utilised the transposon TnphoA to generate Salmonella typhimurium mutants of reduced virulence when given orally to mice (Miller et ai., 1989). S. typhimurium 014 and 046 are two such attenuated derivatiees of the mouse-virulent strain C5, being approximately 3-6 orders of magnitude less virulent than the parent. They were originally thought to have different properties (Miller et al., 1989), but subsequent analysis revealed them to the identical (our unpublished observations). Both mutants were insensitive to the cytolytic effects of serum plus complement and were unaltered in ,~their ability to invade. MDCK cell monolayers (B. Fmlay," pets. comm.). In vivo, ,ae mutants localized in the livers and spleens of infected mice, whether administered by intravenous or oral routes, though the number of bacteria that could be recow~red from infected tissues was ca. 105-fold lower than observed with the parent strain. In this work we further characterize the lesion in these mutants.
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