Combined imaging of bacteria and oxygen in biofilms

Publication Type:
Journal Article
Citation:
Applied and Environmental Microbiology, 2007, 73 (19), pp. 6289 - 6295
Issue Date:
2007-10-01
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Transparent sensors for microscopic O2 imaging were developed by spin coating an ultrathin (<1- to 2-μM) layer of a luminescent O 2 indicator onto coverslips. The sensors showed (i) an ideal Stern-Volmer quenching behavior of the luminescence lifetime towards O 2 levels, (ii) homogeneous measuring characteristics over the sensor surface, and (iii) a linear decline of luminescence lifetime with increasing temperature. When a batch of such coverslip sensors has been characterized, their use is thus essentially calibration free at a known temperature. The sensors are easy to use in flow chambers and other growth devices used in microbiology. We present the first application for combined imaging of O 2 and bacteria in a biofilm flow chamber mounted on a microscope equipped with a spinning-disk confocal unit and a luminescence lifetime camera system. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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