Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Chen, F; Li, K; Hart-Smith, G; Xu, YD; Jiang, Y; Lu, H; Fok, S; Macmillian, A; Pandzic, E; Stenzel, M

Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Chen, F Li, K Hart-Smith, G Xu, YD Jiang, Y Lu, H

Fok, S Macmillian, A Pandzic, E Stenzel, M

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Publication Type:: Journal Article
Citation:: Chemical Communications, 2018, 54 (89), pp. 12618 - 12621
Issue Date:: 2018-01-01

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Field	Value	Language
dc.contributor.author	Chen, F	en_US
dc.contributor.author	Li, K	en_US
dc.contributor.author	Hart-Smith, G	en_US
dc.contributor.author	Xu, YD	en_US
dc.contributor.author	Jiang, Y	en_US
dc.contributor.author	Lu, H https://orcid.org/0000-0001-6932-1900	en_US
dc.contributor.author	Fok, S	en_US
dc.contributor.author	Macmillian, A	en_US
dc.contributor.author	Pandzic, E	en_US
dc.contributor.author	Stenzel, M	en_US
dc.date.issued	2018-01-01	en_US
dc.identifier.citation	Chemical Communications, 2018, 54 (89), pp. 12618 - 12621	en_US
dc.identifier.issn	1359-7345	en_US
dc.identifier.uri	http://hdl.handle.net/10453/133106
dc.description.abstract	© The Royal Society of Chemistry. Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.	en_US
dc.relation.ispartof	Chemical Communications	en_US
dc.relation.isbasedon	10.1039/C8CC04986F	en_US
dc.subject.classification	Organic Chemistry	en_US
dc.subject.mesh	Spheroids, Cellular	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Ions	en_US
dc.subject.mesh	Polymers	en_US
dc.subject.mesh	Muramidase	en_US
dc.subject.mesh	Drug Carriers	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Drug Delivery Systems	en_US
dc.subject.mesh	Molecular Structure	en_US
dc.subject.mesh	Micelles	en_US
dc.subject.mesh	Static Electricity	en_US
dc.subject.mesh	MCF-7 Cells	en_US
dc.title	Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery	en_US
dc.type	Journal Article
utslib.citation.volume	89	en_US
utslib.citation.volume	54	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	03 Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	89	en_US
pubs.publication-status	Published	en_US
pubs.volume	54	en_US

Abstract:

© The Royal Society of Chemistry. Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/133106