A nitrite microsensor for profiling environmental biofilms

De Beer, D; Schramm, A; Santegoeds, CM; Kühl, M

A nitrite microsensor for profiling environmental biofilms

De Beer, D Schramm, A Santegoeds, CM Kühl, M

Permalink

Publication Type:: Journal Article
Citation:: Applied and Environmental Microbiology, 1997, 63 (3), pp. 973 - 977
Issue Date:: 1997-03-01

Closed Access

	Filename	Description	Size
	2009005333OK.pdf		91.87 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	De Beer, D	en_US
dc.contributor.author	Schramm, A	en_US
dc.contributor.author	Santegoeds, CM	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.date.issued	1997-03-01	en_US
dc.identifier.citation	Applied and Environmental Microbiology, 1997, 63 (3), pp. 973 - 977	en_US
dc.identifier.issn	0099-2240	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13326
dc.description.abstract	A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyano-cobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 μm. The response is log-linear in freshwater down to 1 μM NO2- and in seawater to 10 μM NO2-. A method is described for preparation of relatively large polyvinyl chloride (PVC)-gelled liquid membrane microsensors with a tip diameter of 5 to 15 μm, having a hydrophilic coating on the tip. The coating and increased tip diameter resulted in more sturdy sensors, with a lower detection limit and a more stable signal than uncoated nitrite sensors with a tip diameter of 1 to 3 μm. The coating protects the sensor membrane from detrimental direct contact with biomass and can be used for all PVC-gelled liquid membrane sensors meant for profiling microbial mats, biofilms, and sediments. Thanks to these improvements, liquid membrane sensors can now be used in complex environmental samples and in situ, e.g., in operating bioreactors. Examples of measurements in denitrifying, nitrifying, and nitrifying/denitrifying biofilms from wastewater treatment plants are shown. In all of these biofilms high nitrite concentrations were found in narrow zones of less than 1 mm.	en_US
dc.relation.ispartof	Applied and Environmental Microbiology	en_US
dc.subject.classification	Microbiology	en_US
dc.title	A nitrite microsensor for profiling environmental biofilms	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	63	en_US
utslib.for	0605 Microbiology	en_US
dc.location.activity	Lahore, Pakistan
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	63	en_US

Abstract:

A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyano-cobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 μm. The response is log-linear in freshwater down to 1 μM NO2- and in seawater to 10 μM NO2-. A method is described for preparation of relatively large polyvinyl chloride (PVC)-gelled liquid membrane microsensors with a tip diameter of 5 to 15 μm, having a hydrophilic coating on the tip. The coating and increased tip diameter resulted in more sturdy sensors, with a lower detection limit and a more stable signal than uncoated nitrite sensors with a tip diameter of 1 to 3 μm. The coating protects the sensor membrane from detrimental direct contact with biomass and can be used for all PVC-gelled liquid membrane sensors meant for profiling microbial mats, biofilms, and sediments. Thanks to these improvements, liquid membrane sensors can now be used in complex environmental samples and in situ, e.g., in operating bioreactors. Examples of measurements in denitrifying, nitrifying, and nitrifying/denitrifying biofilms from wastewater treatment plants are shown. In all of these biofilms high nitrite concentrations were found in narrow zones of less than 1 mm.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/13326