Molecular cloning and structural organization of the human inducible nitric oxide synthase gene (NOS2).
- Publication Type:
- Journal Article
- Citation:
- Biochem Biophys Res Commun, 1996, 219 (3), pp. 784 - 788
- Issue Date:
- 1996-02-27
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Previously, we reported the isolation and molecular cloning of human inducible nitric oxide synthase gene (NOS2) sequences from human chromosome 17 cosmid libraries. Here we describe the further characterization and sequencing of the NOS2 gene. The genomic structure of the NOS2 gene was determined from two overlapping cosmid clones, namely, pcos4A and pcos20. the NOS2 open reading frame is encoded by 27 exons, with translation initiation and termination in exon 2 and exon 27, respectively. These results differ from the previously reported organization of the iNOS gene, where 26 exons were reported for the genomic structure of NOS2.
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