MMP-11 as a biomarker for metastatic breast cancer by immunohistochemical-assisted imaging mass spectrometry

González de Vega, R; Clases, D; Fernández-Sánchez, ML; Eiró, N; González, LO; Vizoso, FJ; Doble, PA; Sanz-Medel, A

MMP-11 as a biomarker for metastatic breast cancer by immunohistochemical-assisted imaging mass spectrometry

González de Vega, R

Clases, D

Fernández-Sánchez, ML Eiró, N González, LO Vizoso, FJ Doble, PA

Sanz-Medel, A

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Publication Type:: Journal Article
Citation:: Analytical and Bioanalytical Chemistry, 2019, 411 (3), pp. 639 - 646
Issue Date:: 2019-01-23

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Field	Value	Language
dc.contributor.author	González de Vega, R https://orcid.org/0000-0002-9806-9633	en_US
dc.contributor.author	Clases, D https://orcid.org/0000-0003-3880-9385	en_US
dc.contributor.author	Fernández-Sánchez, ML	en_US
dc.contributor.author	Eiró, N	en_US
dc.contributor.author	González, LO	en_US
dc.contributor.author	Vizoso, FJ	en_US
dc.contributor.author	Doble, PA https://orcid.org/0000-0002-8472-1301	en_US
dc.contributor.author	Sanz-Medel, A	en_US
dc.date.available	2018-09-06	en_US
dc.date.issued	2019-01-23	en_US
dc.identifier.citation	Analytical and Bioanalytical Chemistry, 2019, 411 (3), pp. 639 - 646	en_US
dc.identifier.issn	1618-2642	en_US
dc.identifier.uri	http://hdl.handle.net/10453/133692
dc.description.abstract	© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. MMP-11 is a member of the matrix metalloproteinase family (MMPs) which are overexpressed in cancer cells, stromal cells and the adjacent microenvironment. The MMP protein family encompasses zinc-dependent endopeptidases that degrade the extracellular matrix (ECM), facilitating the breakdown of the basal membrane and matrix connective tissues. This function is believed to be important in cancer development and metastasis. This paper investigated a gold nanoparticle-based immunohistochemical assay to visualise the distribution of MMP-11 in human breast cancer tissues from eight patients with and without metastases by employing laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The expression of MMP-11 was increased and more heterogeneous in metastatic specimens compared to non-metastatic tumour samples. These findings demonstrate that imaging breast tumours by LA-ICP-MS may be a useful tool to aid the prognosis and treatment of breast cancer. As an example, samples of two patients are presented who were diagnosed with matching characteristics and grades of breast cancer. Although both patients had a similar prognosis and treatment, only one developed metastases.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP170100036
dc.relation.ispartof	Analytical and Bioanalytical Chemistry	en_US
dc.relation.isbasedon	10.1007/s00216-018-1365-3	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Breast	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Breast Neoplasms	en_US
dc.subject.mesh	Neoplasm Metastasis	en_US
dc.subject.mesh	Gold	en_US
dc.subject.mesh	Immunohistochemistry	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.subject.mesh	Matrix Metalloproteinase 11	en_US
dc.subject.mesh	Metal Nanoparticles	en_US
dc.subject.mesh	Laser Therapy	en_US
dc.subject.mesh	Biomarkers, Tumor	en_US
dc.title	MMP-11 as a biomarker for metastatic breast cancer by immunohistochemical-assisted imaging mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	411	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	411	en_US

Abstract:

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. MMP-11 is a member of the matrix metalloproteinase family (MMPs) which are overexpressed in cancer cells, stromal cells and the adjacent microenvironment. The MMP protein family encompasses zinc-dependent endopeptidases that degrade the extracellular matrix (ECM), facilitating the breakdown of the basal membrane and matrix connective tissues. This function is believed to be important in cancer development and metastasis. This paper investigated a gold nanoparticle-based immunohistochemical assay to visualise the distribution of MMP-11 in human breast cancer tissues from eight patients with and without metastases by employing laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The expression of MMP-11 was increased and more heterogeneous in metastatic specimens compared to non-metastatic tumour samples. These findings demonstrate that imaging breast tumours by LA-ICP-MS may be a useful tool to aid the prognosis and treatment of breast cancer. As an example, samples of two patients are presented who were diagnosed with matching characteristics and grades of breast cancer. Although both patients had a similar prognosis and treatment, only one developed metastases.

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http://hdl.handle.net/10453/133692