High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.

Oxer, MD; Bentley, CM; Doyle, JG; Peakman, TC; Charles, IG; Makoff, AJ

High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.

Oxer, MD Bentley, CM Doyle, JG Peakman, TC Charles, IG Makoff, AJ

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Publication Type:: Journal Article
Citation:: Nucleic Acids Res, 1991, 19 (11), pp. 2889 - 2892
Issue Date:: 1991-06-11

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Field	Value	Language
dc.contributor.author	Oxer, MD	en_US
dc.contributor.author	Bentley, CM	en_US
dc.contributor.author	Doyle, JG	en_US
dc.contributor.author	Peakman, TC	en_US
dc.contributor.author	Charles, IG	en_US
dc.contributor.author	Makoff, AJ	en_US
dc.date.issued	1991-06-11	en_US
dc.identifier.citation	Nucleic Acids Res, 1991, 19 (11), pp. 2889 - 2892	en_US
dc.identifier.issn	0305-1048	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13373
dc.description.abstract	The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels are comparable to those obtained for the same products using the tac promoter. The nirB promoter is very well regulated, giving almost two orders of magnitude increase in fragment C on complete removal of oxygen. The use of this anaerobically-induced promoter in the production of recombinant proteins in E. coli is discussed.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Nucleic Acids Res	en_US
dc.relation.isbasedon	10.1093/nar/19.11.2889	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Bordetella pertussis	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Recombinant Proteins	en_US
dc.subject.mesh	Electrophoresis, Polyacrylamide Gel	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Anaerobiosis	en_US
dc.subject.mesh	Fermentation	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Promoter Regions, Genetic	en_US
dc.title	High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	19	en_US
utslib.location.activity	England	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
utslib.for	0604 Genetics	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	19	en_US

Abstract:

The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels are comparable to those obtained for the same products using the tac promoter. The nirB promoter is very well regulated, giving almost two orders of magnitude increase in fragment C on complete removal of oxygen. The use of this anaerobically-induced promoter in the production of recombinant proteins in E. coli is discussed.

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http://hdl.handle.net/10453/13373