Division plane placement in pleomorphic archaea is dynamically coupled to cell shape

Walsh, JC; Angstmann, CN; Bisson-Filho, AW; Garner, EC; Duggin, IG; Curmi, PMG

Division plane placement in pleomorphic archaea is dynamically coupled to cell shape

Walsh, JC Angstmann, CN Bisson-Filho, AW Garner, EC Duggin, IG

Curmi, PMG

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Publication Type:: Journal Article
Citation:: Molecular Microbiology, 2019, 112 (3), pp. 785 - 799
Issue Date:: 2019-09-01

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Field	Value	Language
dc.contributor.author	Walsh, JC	en_US
dc.contributor.author	Angstmann, CN	en_US
dc.contributor.author	Bisson-Filho, AW	en_US
dc.contributor.author	Garner, EC	en_US
dc.contributor.author	Duggin, IG https://orcid.org/0000-0003-4868-7350	en_US
dc.contributor.author	Curmi, PMG https://orcid.org/0000-0001-5762-7638	en_US
dc.date.available	2020-05-28T19:09:41Z
dc.date.issued	2019-09-01	en_US
dc.identifier.citation	Molecular Microbiology, 2019, 112 (3), pp. 785 - 799	en_US
dc.identifier.issn	0950-382X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/133769
dc.description.abstract	© 2019 John Wiley & Sons Ltd One mechanism for achieving accurate placement of the cell division machinery is via Turing patterns, where nonlinear molecular interactions spontaneously produce spatiotemporal concentration gradients. The resulting patterns are dictated by cell shape. For example, the Min system of Escherichia coli shows spatiotemporal oscillation between cell poles, leaving a mid-cell zone for division. The universality of pattern-forming mechanisms in divisome placement is currently unclear. We examined the location of the division plane in two pleomorphic archaea, Haloferax volcanii and Haloarcula japonica, and showed that it correlates with the predictions of Turing patterning. Time-lapse analysis of H. volcanii shows that divisome locations after successive rounds of division are dynamically determined by daughter cell shape. For H. volcanii, we show that the location of DNA does not influence division plane location, ruling out nucleoid occlusion. Triangular cells provide a stringent test for Turing patterning, where there is a bifurcation in division plane orientation. For the two archaea examined, most triangular cells divide as predicted by a Turing mechanism; however, in some cases multiple division planes are observed resulting in cells dividing into three viable progeny. Our results suggest that the division site placement is consistent with a Turing patterning system in these archaea.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP160101076
dc.relation	http://purl.org/au-research/grants/arc/FT160100010
dc.relation.ispartof	Molecular Microbiology	en_US
dc.relation.isbasedon	10.1111/mmi.14316	en_US
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Microbiology	en_US
dc.title	Division plane placement in pleomorphic archaea is dynamically coupled to cell shape	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	112	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
utslib.for	0701 Agriculture, Land and Farm Management	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access	*
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	112	en_US

Abstract:

© 2019 John Wiley & Sons Ltd One mechanism for achieving accurate placement of the cell division machinery is via Turing patterns, where nonlinear molecular interactions spontaneously produce spatiotemporal concentration gradients. The resulting patterns are dictated by cell shape. For example, the Min system of Escherichia coli shows spatiotemporal oscillation between cell poles, leaving a mid-cell zone for division. The universality of pattern-forming mechanisms in divisome placement is currently unclear. We examined the location of the division plane in two pleomorphic archaea, Haloferax volcanii and Haloarcula japonica, and showed that it correlates with the predictions of Turing patterning. Time-lapse analysis of H. volcanii shows that divisome locations after successive rounds of division are dynamically determined by daughter cell shape. For H. volcanii, we show that the location of DNA does not influence division plane location, ruling out nucleoid occlusion. Triangular cells provide a stringent test for Turing patterning, where there is a bifurcation in division plane orientation. For the two archaea examined, most triangular cells divide as predicted by a Turing mechanism; however, in some cases multiple division planes are observed resulting in cells dividing into three viable progeny. Our results suggest that the division site placement is consistent with a Turing patterning system in these archaea.

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http://hdl.handle.net/10453/133769