Salmonella genomic island 1B variant found in a sequence type 117 avian pathogenic Escherichia coli isolate

Cummins, ML; Chowdhury, PR; Marenda, MS; Browning, GF; Djordjevic, SP

Salmonella genomic island 1B variant found in a sequence type 117 avian pathogenic Escherichia coli isolate

Cummins, ML Chowdhury, PR

Marenda, MS Browning, GF

Djordjevic, SP

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Publication Type:: Journal Article
Citation:: mSphere, 2019, 4 (3)
Issue Date:: 2019-05-01

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Field	Value	Language
dc.contributor.author	Cummins, ML	en_US
dc.contributor.author	Chowdhury, PR https://orcid.org/0000-0003-4352-603X	en_US
dc.contributor.author	Marenda, MS	en_US
dc.contributor.author	Browning, GF https://orcid.org/0000-0002-0903-2469	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.date.issued	2019-05-01	en_US
dc.identifier.citation	mSphere, 2019, 4 (3)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/133819
dc.description.abstract	© 2019 Cummins et al. Salmonella genomic island 1 (SGI1) is an integrative genetic island first described in Salmonella enterica serovars Typhimurium DT104 and Agona in 2000. Variants of it have since been described in multiple serovars of S. enterica, as well as in Proteus mirabilis, Acinetobacter baumannii, Morganella morganii, and several other genera. The island typically confers resistance to older, first-generation antimicrobials; however, some variants carry blaNDM-1, blaVEB-6, and blaCTX-M15 genes that encode resistance to frontline, clinically important antibiotics, including third-generation cephalosporins. Genome sequencing studies of avian pathogenic Escherichia coli (APEC) identified a sequence type 117 (ST117) isolate (AVC96) with genetic features found in SGI1. The complete genome sequence of AVC96 was assembled from a combination of Illumina and single-molecule real-time (SMRT) sequence data. Analysis of the AVC96 chromosome identified a variant of SGI1-B located 18 bp from the 3= end of trmE, also known as the attB site, a known hot spot for the integration of genomic islands. This is the first report of SGI1 in wild-type E. coli. The variant, here named SGI1-B-Ec1, was otherwise unremarkable, apart from the identification of ISEc43 in open reading frame (ORF) S023.	en_US
dc.relation.ispartof	mSphere	en_US
dc.relation.isbasedon	10.1128/mSphere.00169-19	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Birds	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Drug Resistance, Bacterial	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Gene Transfer, Horizontal	en_US
dc.subject.mesh	Genomic Islands	en_US
dc.subject.mesh	Salmonella enterica	en_US
dc.subject.mesh	Whole Genome Sequencing	en_US
dc.title	Salmonella genomic island 1B variant found in a sequence type 117 avian pathogenic Escherichia coli isolate	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	4	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	4	en_US

Abstract:

© 2019 Cummins et al. Salmonella genomic island 1 (SGI1) is an integrative genetic island first described in Salmonella enterica serovars Typhimurium DT104 and Agona in 2000. Variants of it have since been described in multiple serovars of S. enterica, as well as in Proteus mirabilis, Acinetobacter baumannii, Morganella morganii, and several other genera. The island typically confers resistance to older, first-generation antimicrobials; however, some variants carry blaNDM-1, blaVEB-6, and blaCTX-M15 genes that encode resistance to frontline, clinically important antibiotics, including third-generation cephalosporins. Genome sequencing studies of avian pathogenic Escherichia coli (APEC) identified a sequence type 117 (ST117) isolate (AVC96) with genetic features found in SGI1. The complete genome sequence of AVC96 was assembled from a combination of Illumina and single-molecule real-time (SMRT) sequence data. Analysis of the AVC96 chromosome identified a variant of SGI1-B located 18 bp from the 3= end of trmE, also known as the attB site, a known hot spot for the integration of genomic islands. This is the first report of SGI1 in wild-type E. coli. The variant, here named SGI1-B-Ec1, was otherwise unremarkable, apart from the identification of ISEc43 in open reading frame (ORF) S023.

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http://hdl.handle.net/10453/133819