Isolation And Computer-Aided Characterization Of Mmei, A Type-Ii Restriction Endonuclease From Methylophilus-Methylotrophus

Oxford Univ Press United Kingdom
Publication Type:
Journal Article
Nucleic Acids Research, 1986, 14 (13), pp. 5255 - 5274
Issue Date:
Full metadata record
Files in This Item:
Filename Description SizeFormat
2009002875OK.pdf2.29 MBAdobe PDF
A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a two nucleotide 3' projection. Determination of the recognition sequence was achieved using two new computer programs; RECOG, which predicts recognition sequences from the pattern of restriction fragments obtained from DNAs of known sequence, and GELSIM, which generates graphical simulations of DNA band patterns obtained by gel electrophoresis of restriction digests of sequenced DNA molecules.
Please use this identifier to cite or link to this item: