Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid

Venturini, C; Beatson, SA; Djordjevic, SP; Walker, MJ

Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid

Venturini, C Beatson, SA Djordjevic, SP

Walker, MJ

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Publication Type:: Journal Article
Citation:: FASEB Journal, 2010, 24 (4), pp. 1160 - 1166
Issue Date:: 2010-04-01

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Field	Value	Language
dc.contributor.author	Venturini, C	en_US
dc.contributor.author	Beatson, SA	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Walker, MJ	en_US
dc.date.issued	2010-04-01	en_US
dc.identifier.citation	FASEB Journal, 2010, 24 (4), pp. 1160 - 1166	en_US
dc.identifier.issn	0892-6638	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13393
dc.description.abstract	Enterohemorrhagic Escherichia coli (EHEC) strains are zoonotic pathogens responsible for a range of severe human disease. The repertoire of virulence determinants promoting EHEC disease is encoded on both the main chromosome and virulence plasmid. We examined a multiply antibiotic-resistant O26 EHEC strain for carriage of resistance genes on the virulence plasmid. The EHEC virulence plasmid containing a complex antibiotic-resistance gene locus, designated as pO26-CRL, was purified from EHEC O26:H- (patient with hemorrhagic colitis) and subjected to shotgun-sequencing and bioinformatic analysis. Determination of the 111,481-bp sequence of pO26-CRL revealed genes encoding a functional enterohemolysin operon (ehxCABD), STEC-specific extracellular serine protease (espP), putative EHEC adhesin (toxB), catalase/peroxidase (katP), and myristoyl transferase (msbB) involved in lipid A synthesis. A 22,609-bp Tn21 derivative is inserted within the conjugal transfer gene traC and encodes resistance to trimethoprim, streptomycin, sulfathiozole, kanamycin, neomycin, β-lactams, and mercuric chloride. Plasmid pO26-CRL is nonconjugative but is mobilizable. This is the first report of an EHEC virulence plasmid containing a complex antibiotic resistance locus, and raises the concern that antibiotic use will coselect for virulence determinants, leading to increased disease potential in both commensal and pathogenic E. coli populations. © FASEB.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LP0348851
dc.relation.ispartof	FASEB Journal	en_US
dc.relation.isbasedon	10.1096/fj.09-144972	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Escherichia coli Infections	en_US
dc.subject.mesh	Catalase	en_US
dc.subject.mesh	Acyltransferases	en_US
dc.subject.mesh	Escherichia coli Proteins	en_US
dc.subject.mesh	Drug Resistance, Multiple, Bacterial	en_US
dc.subject.mesh	Operon	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Hemolysin Proteins	en_US
dc.subject.mesh	Enterohemorrhagic Escherichia coli	en_US
dc.subject.mesh	Genetic Loci	en_US
dc.title	Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	24	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0606 Physiology	en_US
utslib.for	1116 Medical Physiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	24	en_US

Abstract:

Enterohemorrhagic Escherichia coli (EHEC) strains are zoonotic pathogens responsible for a range of severe human disease. The repertoire of virulence determinants promoting EHEC disease is encoded on both the main chromosome and virulence plasmid. We examined a multiply antibiotic-resistant O26 EHEC strain for carriage of resistance genes on the virulence plasmid. The EHEC virulence plasmid containing a complex antibiotic-resistance gene locus, designated as pO26-CRL, was purified from EHEC O26:H- (patient with hemorrhagic colitis) and subjected to shotgun-sequencing and bioinformatic analysis. Determination of the 111,481-bp sequence of pO26-CRL revealed genes encoding a functional enterohemolysin operon (ehxCABD), STEC-specific extracellular serine protease (espP), putative EHEC adhesin (toxB), catalase/peroxidase (katP), and myristoyl transferase (msbB) involved in lipid A synthesis. A 22,609-bp Tn21 derivative is inserted within the conjugal transfer gene traC and encodes resistance to trimethoprim, streptomycin, sulfathiozole, kanamycin, neomycin, β-lactams, and mercuric chloride. Plasmid pO26-CRL is nonconjugative but is mobilizable. This is the first report of an EHEC virulence plasmid containing a complex antibiotic resistance locus, and raises the concern that antibiotic use will coselect for virulence determinants, leading to increased disease potential in both commensal and pathogenic E. coli populations. © FASEB.

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http://hdl.handle.net/10453/13393