Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi.
- Publication Type:
- Journal Article
- Citation:
- J Gen Microbiol, 1991, 137 (1), pp. 147 - 152
- Issue Date:
- 1991-01
Closed Access
| Filename | Description | Size | |||
|---|---|---|---|---|---|
 | 2009004802OK.pdf | 1 MB |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
The aroD gene from Salmonella typhi, encoding 5-dehydroquinate hydrolyase (3-dehydroquinase), has been cloned into Escherichia coli and the DNA sequence determined. The aroD gene was isolated from a cosmid gene bank by complementation of an S. typhimurium aroD mutant. Analysis of the DNA sequence revealed the presence of an open reading frame capable of encoding a protein of 252 amino acids with a calculated Mr of 27706. Comparison of the deduced S. typhi 3-dehydroquinase protein sequence with that elucidated for E. coli revealed 69% homology. Alignment of the S. typhi sequence and equivalent Aspergillus nidulans and Saccharomyces cerevisiae sequences showed that homology was lower, at 24%, but still significant. Use of a minicell expression system demonstrated that a polyclonal antibody raised against E. coli 3-dehydroquinase cross-related with its S. typhi counterpart.
Please use this identifier to cite or link to this item: