Advances in single particle tracking in living cells

Publication Type:
Journal Article
Chinese Optics, 2018, 11 (3), pp. 281 - 295
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© 2018, China Science Publishing & Media LTD. All right reserved. Single particle tracking(SPT) technique locates and tracks individual fluorescent or scattering particles within a cell with the help of microscope system. Based on the ability of real-time monitoring of the complex and highly dynamic changes in tissue structure within living cells and the ability to provide dynamic relationships between structure and function, SPT has important applications in cell biology. In this review,the mechanism of SPT and its application on cells are summarized. Firstly, the dynamics of SPT are introduced, including single particle localization, trajectory reconstruction and analysis. Then the optical materials and instruments that SPT technology focuses on at the present stage are described. Finally, the application of SPT in cell membrane, intracellular signaling pathway, molecular transport mechanism, genetic information expression, and viral infection mechanism are proposed. In addition, the advance of SPT technology are prospected in this paper.
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