Diel variations in carbon metabolism by green nonsulfur-like bacteria in alkaline siliceous hot spring microbial mats from Yellowstone National Park

Van Der Meer, MTJ; Schouten, S; Bateson, MM; Nübel, U; Wieland, A; Kühl, M; De Leeuw, JW; Damsté, JSS; Ward, DM

Diel variations in carbon metabolism by green nonsulfur-like bacteria in alkaline siliceous hot spring microbial mats from Yellowstone National Park

Van Der Meer, MTJ Schouten, S Bateson, MM Nübel, U Wieland, A Kühl, M

De Leeuw, JW Damsté, JSS Ward, DM

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Publication Type:: Journal Article
Citation:: Applied and Environmental Microbiology, 2005, 71 (7), pp. 3978 - 3986
Issue Date:: 2005-07-01

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Field	Value	Language
dc.contributor.author	Van Der Meer, MTJ	en_US
dc.contributor.author	Schouten, S	en_US
dc.contributor.author	Bateson, MM	en_US
dc.contributor.author	Nübel, U	en_US
dc.contributor.author	Wieland, A	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	De Leeuw, JW	en_US
dc.contributor.author	Damsté, JSS	en_US
dc.contributor.author	Ward, DM	en_US
dc.date.issued	2005-07-01	en_US
dc.identifier.citation	Applied and Environmental Microbiology, 2005, 71 (7), pp. 3978 - 3986	en_US
dc.identifier.issn	0099-2240	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13408
dc.description.abstract	Green nonsulfur-like bacteria (GNSLB) in hot spring microbial mats are thought to be mainly photoheterotrophic, using cyanobacterial metabolites as carbon sources. However, the stable carbon isotopic composition of typical Chloroflexus and Roseiflexus lipids suggests photoautotrophic metabolism of GNSLB. One possible explanation for this apparent discrepancy might be that GNSLB fix inorganic carbon only during certain times of the day. In order to study temporal variability in carbon metabolism by GNSLB, labeling experiments with [13C]bicarbonate, [14C]bicarbonate, and [ 13C]acetate were performed during different times of the day. [ 14C]bicarbonate labeling indicated that during the morning, incorporation of label was light dependent and that both cyanobacteria and GNSLB were involved in bicarbonate uptake. 13C-labeling experiments indicated that during the morning, GNSLB incorporated labeled bicarbonate at least to the same degree as cyanobacteria. The incorporation of [13C] bicarbonate into specific lipids could be stimulated by the addition of sulfide or hydrogen, which both were present in the morning photic zone. The results suggest that GNSLB have the potential for photoautotrophic metabolism during low-light periods. In high-light periods, inorganic carbon was incorporated primarily into Cyanobacteria-specific lipids. The results of a pulse-labeling experiment were consistent with overnight transfer of label to GNSLB, which could be interrupted by the addition of unlabeled acetate and glycolate. In addition, we observed direct incorporation of [13C]acetate into GNSLB lipids in the morning. This suggests that GNSLB also have a potential for photoheterotrophy in situ. Copyright © 2005, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Applied and Environmental Microbiology	en_US
dc.relation.isbasedon	10.1128/AEM.71.7.3978-3986.2005	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Chloroflexi	en_US
dc.subject.mesh	Synechococcus	en_US
dc.subject.mesh	Hydrogen Sulfide	en_US
dc.subject.mesh	Carbon	en_US
dc.subject.mesh	Carbon Isotopes	en_US
dc.subject.mesh	Carbon Radioisotopes	en_US
dc.subject.mesh	Hot Springs	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.subject.mesh	Light	en_US
dc.subject.mesh	Lipid Metabolism	en_US
dc.title	Diel variations in carbon metabolism by green nonsulfur-like bacteria in alkaline siliceous hot spring microbial mats from Yellowstone National Park	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	71	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	71	en_US

Abstract:

Green nonsulfur-like bacteria (GNSLB) in hot spring microbial mats are thought to be mainly photoheterotrophic, using cyanobacterial metabolites as carbon sources. However, the stable carbon isotopic composition of typical Chloroflexus and Roseiflexus lipids suggests photoautotrophic metabolism of GNSLB. One possible explanation for this apparent discrepancy might be that GNSLB fix inorganic carbon only during certain times of the day. In order to study temporal variability in carbon metabolism by GNSLB, labeling experiments with [13C]bicarbonate, [14C]bicarbonate, and [ 13C]acetate were performed during different times of the day. [ 14C]bicarbonate labeling indicated that during the morning, incorporation of label was light dependent and that both cyanobacteria and GNSLB were involved in bicarbonate uptake. 13C-labeling experiments indicated that during the morning, GNSLB incorporated labeled bicarbonate at least to the same degree as cyanobacteria. The incorporation of [13C] bicarbonate into specific lipids could be stimulated by the addition of sulfide or hydrogen, which both were present in the morning photic zone. The results suggest that GNSLB have the potential for photoautotrophic metabolism during low-light periods. In high-light periods, inorganic carbon was incorporated primarily into Cyanobacteria-specific lipids. The results of a pulse-labeling experiment were consistent with overnight transfer of label to GNSLB, which could be interrupted by the addition of unlabeled acetate and glycolate. In addition, we observed direct incorporation of [13C]acetate into GNSLB lipids in the morning. This suggests that GNSLB also have a potential for photoheterotrophy in situ. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/13408