Characterization of biologic response modifiers in the supernatant of conventional, refrigerated, and cryopreserved platelets

Johnson, L; Tan, S; Jenkins, E; Wood, B; Marks, DC

Characterization of biologic response modifiers in the supernatant of conventional, refrigerated, and cryopreserved platelets

Johnson, L

Tan, S Jenkins, E Wood, B Marks, DC

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Publication Type:: Journal Article
Citation:: Transfusion, 2018, 58 (4), pp. 927 - 937
Issue Date:: 2018-04-01

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Field	Value	Language
dc.contributor.author	Johnson, L https://orcid.org/0000-0003-3303-4437	en_US
dc.contributor.author	Tan, S	en_US
dc.contributor.author	Jenkins, E	en_US
dc.contributor.author	Wood, B	en_US
dc.contributor.author	Marks, DC	en_US
dc.date.available	2017-11-28	en_US
dc.date.issued	2018-04-01	en_US
dc.identifier.citation	Transfusion, 2018, 58 (4), pp. 927 - 937	en_US
dc.identifier.issn	0041-1132	en_US
dc.identifier.uri	http://hdl.handle.net/10453/134279
dc.description.abstract	© 2017 AABB BACKGROUND: Alternatives to room temperature storage of platelets (PLTs) are of interest to support blood banking logistics. The aim of this study was to compare the presence of biologic response modifiers (BRMs) in PLT concentrates stored under conventional room temperature conditions with refrigerated or cryopreserved PLTs. STUDY DESIGN AND METHODS: A three-arm pool-and-split study was carried out using buffy coat–derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were as follows: room temperature (20-24°C), cold (2-6°C), and cryopreserved (–80°C with DMSO). Liquid-stored PLTs were tested over a 21-day period, while cryopreserved PLTs were tested immediately after thawing and reconstitution in 30% plasma/70% SSP+ and after storage at room temperature. RESULTS: Coagulation factor activity was comparable between room temperature and cold PLTs, with the exception of protein S, while cryopreserved PLTs had reduced Factor (F)V and FVIII activity. Cold-stored PLTs retained α-granule proteins better than room temperature or cryopreserved PLTs. Cryopreservation resulted in 10-fold higher microparticle generation than cold-stored PLTs, but both groups contained significantly more microparticles than those stored at room temperature. The supernatant from both cold and cryopreserved PLTs initiated faster clot formation and thrombin generation than room temperature PLTs. CONCLUSION: Cold storage and cryopreservation alter the composition of the soluble fraction of stored PLTs. These differences in coagulation proteins, cytokines, and microparticles likely influence both the hemostatic capacity of the components and the auxiliary functions.	en_US
dc.relation.ispartof	Transfusion	en_US
dc.relation.isbasedon	10.1111/trf.14475	en_US
dc.subject.classification	Cardiovascular System & Hematology	en_US
dc.subject.mesh	Blood Platelets	en_US
dc.subject.mesh	Cytoplasmic Granules	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Serotonin	en_US
dc.subject.mesh	Thrombin	en_US
dc.subject.mesh	Intercellular Signaling Peptides and Proteins	en_US
dc.subject.mesh	Blood Coagulation Factors	en_US
dc.subject.mesh	Immunologic Factors	en_US
dc.subject.mesh	Cytokines	en_US
dc.subject.mesh	Thrombelastography	en_US
dc.subject.mesh	Cryopreservation	en_US
dc.subject.mesh	Refrigeration	en_US
dc.subject.mesh	Blood Preservation	en_US
dc.subject.mesh	Centrifugation	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Platelet Activation	en_US
dc.subject.mesh	Cell-Derived Microparticles	en_US
dc.title	Characterization of biologic response modifiers in the supernatant of conventional, refrigerated, and cryopreserved platelets	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	58	en_US
utslib.for	1102 Cardiorespiratory Medicine and Haematology	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	1107 Immunology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	58	en_US

Abstract:

© 2017 AABB BACKGROUND: Alternatives to room temperature storage of platelets (PLTs) are of interest to support blood banking logistics. The aim of this study was to compare the presence of biologic response modifiers (BRMs) in PLT concentrates stored under conventional room temperature conditions with refrigerated or cryopreserved PLTs. STUDY DESIGN AND METHODS: A three-arm pool-and-split study was carried out using buffy coat–derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were as follows: room temperature (20-24°C), cold (2-6°C), and cryopreserved (–80°C with DMSO). Liquid-stored PLTs were tested over a 21-day period, while cryopreserved PLTs were tested immediately after thawing and reconstitution in 30% plasma/70% SSP+ and after storage at room temperature. RESULTS: Coagulation factor activity was comparable between room temperature and cold PLTs, with the exception of protein S, while cryopreserved PLTs had reduced Factor (F)V and FVIII activity. Cold-stored PLTs retained α-granule proteins better than room temperature or cryopreserved PLTs. Cryopreservation resulted in 10-fold higher microparticle generation than cold-stored PLTs, but both groups contained significantly more microparticles than those stored at room temperature. The supernatant from both cold and cryopreserved PLTs initiated faster clot formation and thrombin generation than room temperature PLTs. CONCLUSION: Cold storage and cryopreservation alter the composition of the soluble fraction of stored PLTs. These differences in coagulation proteins, cytokines, and microparticles likely influence both the hemostatic capacity of the components and the auxiliary functions.

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http://hdl.handle.net/10453/134279