Distribution of class 1 integrons with IS26-mediated deletions in their 3'-conserved segments in Escherichia coli of human and animal origin

Dawes, FE; Kuzevski, A; Bettelheim, KA; Hornitzky, MA; Djordjevic, SP; Walker, MJ

Distribution of class 1 integrons with IS26-mediated deletions in their 3'-conserved segments in Escherichia coli of human and animal origin

Dawes, FE Kuzevski, A Bettelheim, KA Hornitzky, MA Djordjevic, SP

Walker, MJ

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2010, 5 (9), pp. 1 - 6
Issue Date:: 2010-11-01

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Field	Value	Language
dc.contributor.author	Dawes, FE	en_US
dc.contributor.author	Kuzevski, A	en_US
dc.contributor.author	Bettelheim, KA	en_US
dc.contributor.author	Hornitzky, MA	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Walker, MJ	en_US
dc.date.available	2010-08-17	en_US
dc.date.issued	2010-11-01	en_US
dc.identifier.citation	PLoS ONE, 2010, 5 (9), pp. 1 - 6	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13445
dc.description.abstract	Class 1 integrons play a role in the emergence of multi-resistant bacteria by facilitating the recruitment of gene cassettes encoding antibiotic resistance genes. 512 E. coli strains sourced from humans (n = 202), animals (n = 304) and the environment (n = 6) were screened for the presence of the intI1 gene. In 31/79 integron positive E. coli strains, the gene cassette regions could not be PCR amplified using standard primers. DNA sequence analysis of 6 serologically diverse strains revealed atypical integrons harboured the dfrA5 cassette gene and only 24 bp of the integron 3'-conserved segment (CS) remained, due to the insertion of IS26. PCR targeting intI1 and IS26 followed by restriction fragment length polymorphism (RFLP) analysis identified the integron-dfrA5-IS26 element in 27 E. coli strains of bovine origin and 4 strains of human origin. Southern hybridization and transformation studies revealed the integron-dfrA5-IS26 gene arrangement was either chromosomally located or plasmid borne. Plasmid location in 4/9 E. coli strains and PCR linkage of Tn21 transposition genes with the intI1 gene in 20/31 strains, suggests this element is readily disseminated by horizontal transfer. © 2010 Dawes et al.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0012754	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cattle	en_US
dc.subject.mesh	Swine	en_US
dc.subject.mesh	Dogs	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Escherichia coli Infections	en_US
dc.subject.mesh	Cattle Diseases	en_US
dc.subject.mesh	Dog Diseases	en_US
dc.subject.mesh	Swine Diseases	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Gene Deletion	en_US
dc.subject.mesh	Integrons	en_US
dc.subject.mesh	Conserved Sequence	en_US
dc.title	Distribution of class 1 integrons with IS26-mediated deletions in their 3'-conserved segments in Escherichia coli of human and animal origin	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	5	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

Class 1 integrons play a role in the emergence of multi-resistant bacteria by facilitating the recruitment of gene cassettes encoding antibiotic resistance genes. 512 E. coli strains sourced from humans (n = 202), animals (n = 304) and the environment (n = 6) were screened for the presence of the intI1 gene. In 31/79 integron positive E. coli strains, the gene cassette regions could not be PCR amplified using standard primers. DNA sequence analysis of 6 serologically diverse strains revealed atypical integrons harboured the dfrA5 cassette gene and only 24 bp of the integron 3'-conserved segment (CS) remained, due to the insertion of IS26. PCR targeting intI1 and IS26 followed by restriction fragment length polymorphism (RFLP) analysis identified the integron-dfrA5-IS26 element in 27 E. coli strains of bovine origin and 4 strains of human origin. Southern hybridization and transformation studies revealed the integron-dfrA5-IS26 gene arrangement was either chromosomally located or plasmid borne. Plasmid location in 4/9 E. coli strains and PCR linkage of Tn21 transposition genes with the intI1 gene in 20/31 strains, suggests this element is readily disseminated by horizontal transfer. © 2010 Dawes et al.

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http://hdl.handle.net/10453/13445