Cotyledons contribute to plant growth and hybrid vigor in Arabidopsis

Wang, L; Liu, PC; Wu, LM; Tan, J; Peacock, WJ; Dennis, ES

Cotyledons contribute to plant growth and hybrid vigor in Arabidopsis

Wang, L Liu, PC Wu, LM Tan, J Peacock, WJ

Dennis, ES

Permalink

Publication Type:: Journal Article
Citation:: Planta, 2019, 249 (4), pp. 1107 - 1118
Issue Date:: 2019-04-11

Closed Access

	Filename	Description	Size
	Wang2018_Article_CotyledonsContributeToPlantGro.pdf	Submitted Version	10.61 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Wang, L	en_US
dc.contributor.author	Liu, PC	en_US
dc.contributor.author	Wu, LM	en_US
dc.contributor.author	Tan, J	en_US
dc.contributor.author	Peacock, WJ https://orcid.org/0000-0001-7314-9074	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.date.available	2018-12-10	en_US
dc.date.issued	2019-04-11	en_US
dc.identifier.citation	Planta, 2019, 249 (4), pp. 1107 - 1118	en_US
dc.identifier.issn	0032-0935	en_US
dc.identifier.uri	http://hdl.handle.net/10453/135021
dc.description.abstract	© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Main conclusion: In hybrids of Arabidopsis, cotyledons influence the amount and proportion of hybrid vigor in total plant growth. We found Arabidopsis cotyledons are essential for plant growth and in some hybrids for hybrid vigor. In hybrids between C24 and Landsberg erecta (Ler), biomass vigor (heterosis) occurs in the first few days after sowing (DAS), with hybrid cotyledons being larger than those of their parents. C24xLer hybrids are ahead of their parents in activating photosynthesis and auxin pathway genes in cotyledons at 3–4 DAS. “Earliness” is also present in newly emerged C24xLer hybrid leaves. We showed cotyledon removal at 4 DAS caused significant biomass reduction in later growth in hybrids and parental lines. The biomass decrease caused by cotyledon removal can be partially rescued by exogenous sucrose or auxin with different genotypes responding to sucrose and/or auxin differently. Cotyledon removal has different effects on heterosis in different hybrids. After cotyledon removal, in C24xLer hybrids, both growth and heterosis were reduced in similar proportions, but the level of hybrid vigor was reduced as a proportion of growth in C24xColumbia (Col) and ColxLer hybrids. The removal of cotyledons at 4 DAS markedly decreased the level of growth and eliminated the heterotic phenotype of Wassilewskija (Ws)/Ler hybrids. In mutant Ws/Ler hybrids which had a reduced level of photosynthesis in the cotyledons, there was a reduction in plant growth and loss of heterosis. The variation in contribution of cotyledons to heterosis in different hybrids indicates there are multiple pathways to achieve heterotic phenotypes.	en_US
dc.relation.ispartof	Planta	en_US
dc.relation.isbasedon	10.1007/s00425-018-3068-6	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Plant Leaves	en_US
dc.subject.mesh	Cotyledon	en_US
dc.subject.mesh	Indoleacetic Acids	en_US
dc.subject.mesh	Sucrose	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Hybrid Vigor	en_US
dc.subject.mesh	Real-Time Polymerase Chain Reaction	en_US
dc.subject.mesh	Plant Breeding	en_US
dc.title	Cotyledons contribute to plant growth and hybrid vigor in Arabidopsis	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	249	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0703 Crop and Pasture Production	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	249	en_US

Abstract:

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Main conclusion: In hybrids of Arabidopsis, cotyledons influence the amount and proportion of hybrid vigor in total plant growth. We found Arabidopsis cotyledons are essential for plant growth and in some hybrids for hybrid vigor. In hybrids between C24 and Landsberg erecta (Ler), biomass vigor (heterosis) occurs in the first few days after sowing (DAS), with hybrid cotyledons being larger than those of their parents. C24xLer hybrids are ahead of their parents in activating photosynthesis and auxin pathway genes in cotyledons at 3–4 DAS. “Earliness” is also present in newly emerged C24xLer hybrid leaves. We showed cotyledon removal at 4 DAS caused significant biomass reduction in later growth in hybrids and parental lines. The biomass decrease caused by cotyledon removal can be partially rescued by exogenous sucrose or auxin with different genotypes responding to sucrose and/or auxin differently. Cotyledon removal has different effects on heterosis in different hybrids. After cotyledon removal, in C24xLer hybrids, both growth and heterosis were reduced in similar proportions, but the level of hybrid vigor was reduced as a proportion of growth in C24xColumbia (Col) and ColxLer hybrids. The removal of cotyledons at 4 DAS markedly decreased the level of growth and eliminated the heterotic phenotype of Wassilewskija (Ws)/Ler hybrids. In mutant Ws/Ler hybrids which had a reduced level of photosynthesis in the cotyledons, there was a reduction in plant growth and loss of heterosis. The variation in contribution of cotyledons to heterosis in different hybrids indicates there are multiple pathways to achieve heterotic phenotypes.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/135021