Effect of Various Natural Dissolved Organic Carbon on Copper Lability and Toxicity to the Tropical Freshwater Microalga Chlorella sp.

Macoustra, G; Holland, A; Stauber, J; Jolley, DF

Effect of Various Natural Dissolved Organic Carbon on Copper Lability and Toxicity to the Tropical Freshwater Microalga Chlorella sp.

Macoustra, G Holland, A Stauber, J Jolley, DF

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Publication Type:: Journal Article
Citation:: Environmental Science and Technology, 2019, 53 (5), pp. 2768 - 2777
Issue Date:: 2019-03-05

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Field	Value	Language
dc.contributor.author	Macoustra, G	en_US
dc.contributor.author	Holland, A	en_US
dc.contributor.author	Stauber, J	en_US
dc.contributor.author	Jolley, DF https://orcid.org/0000-0003-1028-1603	en_US
dc.date.issued	2019-03-05	en_US
dc.identifier.citation	Environmental Science and Technology, 2019, 53 (5), pp. 2768 - 2777	en_US
dc.identifier.issn	0013-936X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/135219
dc.description.abstract	© 2019 American Chemical Society. This study adds further critical information to the limited body of knowledge on the ameliorative ability of Australian dissolved organic carbon (DOC), reinforcing the importance of DOC source and concentration as significant factors controlling the risk copper poses to organisms in freshwater systems. The ameliorative ability of five unstudied DOCs on the chronic toxicity of copper to the tropical alga Chlorella sp. was compared. Sensitivity to copper varied dramatically; effect concentrations at the 50 percent effect level (EC 50 ) increased by up to 22-fold in the high DOC treatment compared to controls and more than 2-fold between DOCs at the same concentration. The analytical techniques, diffusive gradients in thin films (DGT) and Chelex column, were used to understand whether differences in copper toxicity could be explained by copper lability. Labile copper mirrored the trends seen in the toxicity tests; lability decreased with increasing DOC concentration and varied between DOCs at the same concentration. The equilibrium model, WHAM VII, was also used to better understand the role of the free copper ion on the toxicity observed. Disagreement between EC 50 values derived using the WHAM-predicted free Cu 2+ concentrations and agreement between DGT-labile and the maximum dynamic concentration (cmax dyn ) suggest free copper is not the sole contributor to toxicity and that the source of the specific DOCs also plays a role.	en_US
dc.relation.ispartof	Environmental Science and Technology	en_US
dc.relation.isbasedon	10.1021/acs.est.8b04737	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Chlorella	en_US
dc.subject.mesh	Carbon	en_US
dc.subject.mesh	Copper	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Fresh Water	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Microalgae	en_US
dc.title	Effect of Various Natural Dissolved Organic Carbon on Copper Lability and Toxicity to the Tropical Freshwater Microalga Chlorella sp.	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	53	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0305 Organic Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	53	en_US

Abstract:

© 2019 American Chemical Society. This study adds further critical information to the limited body of knowledge on the ameliorative ability of Australian dissolved organic carbon (DOC), reinforcing the importance of DOC source and concentration as significant factors controlling the risk copper poses to organisms in freshwater systems. The ameliorative ability of five unstudied DOCs on the chronic toxicity of copper to the tropical alga Chlorella sp. was compared. Sensitivity to copper varied dramatically; effect concentrations at the 50 percent effect level (EC 50 ) increased by up to 22-fold in the high DOC treatment compared to controls and more than 2-fold between DOCs at the same concentration. The analytical techniques, diffusive gradients in thin films (DGT) and Chelex column, were used to understand whether differences in copper toxicity could be explained by copper lability. Labile copper mirrored the trends seen in the toxicity tests; lability decreased with increasing DOC concentration and varied between DOCs at the same concentration. The equilibrium model, WHAM VII, was also used to better understand the role of the free copper ion on the toxicity observed. Disagreement between EC 50 values derived using the WHAM-predicted free Cu 2+ concentrations and agreement between DGT-labile and the maximum dynamic concentration (cmax dyn ) suggest free copper is not the sole contributor to toxicity and that the source of the specific DOCs also plays a role.

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http://hdl.handle.net/10453/135219