High-throughput Quant-iT PicoGreen assay using an automated liquid handling system

Anantanawat, K; Pitsch, N; Fromont, C; Janitz, C

High-throughput Quant-iT PicoGreen assay using an automated liquid handling system

Anantanawat, K

Pitsch, N Fromont, C Janitz, C

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Publication Type:: Journal Article
Citation:: BioTechniques, 2019, 66 (6), pp. 290 - 294
Issue Date:: 2019-01-01

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Field	Value	Language
dc.contributor.author	Anantanawat, K https://orcid.org/0000-0002-7862-0969	en_US
dc.contributor.author	Pitsch, N	en_US
dc.contributor.author	Fromont, C	en_US
dc.contributor.author	Janitz, C	en_US
dc.date.issued	2019-01-01	en_US
dc.identifier.citation	BioTechniques, 2019, 66 (6), pp. 290 - 294	en_US
dc.identifier.issn	0736-6205	en_US
dc.identifier.uri	http://hdl.handle.net/10453/135421
dc.description.abstract	© 2019 Future Science. All rights reserved. Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid concentrations, which can be achieved using fluorescent dye-based nucleic acid measurement. Here, we report a protocol for preparing a 384-well Quant-iT PicoGreen assay. The protocol allows the concentrations of 184 DNA samples to be measured simultaneously in duplicate in only 1 h using an Eppendorf epMotion 5075 liquid handling system. The advantages of this high-throughput approach include a reduction in both reagents (10x less reagents compared to a standard protocol) and time (3 h for 384 samples compared with 3 days).	en_US
dc.relation.ispartof	BioTechniques	en_US
dc.relation.isbasedon	10.2144/btn-2018-0172	en_US
dc.subject.classification	Bioinformatics	en_US
dc.subject.mesh	Organic Chemicals	en_US
dc.subject.mesh	DNA	en_US
dc.subject.mesh	Fluorescent Dyes	en_US
dc.subject.mesh	Gene Library	en_US
dc.subject.mesh	Workflow	en_US
dc.subject.mesh	High-Throughput Nucleotide Sequencing	en_US
dc.title	High-throughput Quant-iT PicoGreen assay using an automated liquid handling system	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	66	en_US
utslib.for	0604 Genetics	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	66	en_US

Abstract:

© 2019 Future Science. All rights reserved. Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid concentrations, which can be achieved using fluorescent dye-based nucleic acid measurement. Here, we report a protocol for preparing a 384-well Quant-iT PicoGreen assay. The protocol allows the concentrations of 184 DNA samples to be measured simultaneously in duplicate in only 1 h using an Eppendorf epMotion 5075 liquid handling system. The advantages of this high-throughput approach include a reduction in both reagents (10x less reagents compared to a standard protocol) and time (3 h for 384 samples compared with 3 days).

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http://hdl.handle.net/10453/135421