Droplet-based single cell RNAseq tools: A practical guide

Salomon, R; Kaczorowski, D; Valdes-Mora, F; Nordon, RE; Neild, A; Farbehi, N; Bartonicek, N; Gallego-Ortega, D

Droplet-based single cell RNAseq tools: A practical guide

Salomon, R

Kaczorowski, D Valdes-Mora, F Nordon, RE Neild, A Farbehi, N Bartonicek, N Gallego-Ortega, D

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Publication Type:: Journal Article
Citation:: Lab on a Chip, 2019, 19 (10), pp. 1706 - 1727
Issue Date:: 2019-01-01

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Field	Value	Language
dc.contributor.author	Salomon, R https://orcid.org/0000-0003-4504-2901	en_US
dc.contributor.author	Kaczorowski, D	en_US
dc.contributor.author	Valdes-Mora, F	en_US
dc.contributor.author	Nordon, RE	en_US
dc.contributor.author	Neild, A	en_US
dc.contributor.author	Farbehi, N	en_US
dc.contributor.author	Bartonicek, N	en_US
dc.contributor.author	Gallego-Ortega, D	en_US
dc.date.issued	2019-01-01	en_US
dc.identifier.citation	Lab on a Chip, 2019, 19 (10), pp. 1706 - 1727	en_US
dc.identifier.issn	1473-0197	en_US
dc.identifier.uri	http://hdl.handle.net/10453/135796
dc.description.abstract	© 2019 The Royal Society of Chemistry. Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.	en_US
dc.relation.ispartof	Lab on a Chip	en_US
dc.relation.isbasedon	10.1039/c8lc01239c	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	RNA	en_US
dc.subject.mesh	Particle Size	en_US
dc.subject.mesh	Surface Properties	en_US
dc.subject.mesh	Single-Cell Analysis	en_US
dc.subject.mesh	RNA-Seq	en_US
dc.title	Droplet-based single cell RNAseq tools: A practical guide	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	19	en_US
utslib.for	0604 Genetics	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	19	en_US

Abstract:

© 2019 The Royal Society of Chemistry. Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/135796