Vascular protection by ethanol extract of Morus alba root bark: Endothelium-dependent relaxation of rat aorta and decrease of smooth muscle cell migration and proliferation

Panth, N; Paudel, KR; Gong, DS; Oak, MH

Vascular protection by ethanol extract of Morus alba root bark: Endothelium-dependent relaxation of rat aorta and decrease of smooth muscle cell migration and proliferation

Panth, N Paudel, KR

Gong, DS Oak, MH

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Publication Type:: Journal Article
Citation:: Evidence-based Complementary and Alternative Medicine, 2018, 2018
Issue Date:: 2018-01-01

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Field	Value	Language
dc.contributor.author	Panth, N	en_US
dc.contributor.author	Paudel, KR https://orcid.org/0000-0002-3591-2080	en_US
dc.contributor.author	Gong, DS	en_US
dc.contributor.author	Oak, MH	en_US
dc.date.available	2018-10-23	en_US
dc.date.issued	2018-01-01	en_US
dc.identifier.citation	Evidence-based Complementary and Alternative Medicine, 2018, 2018	en_US
dc.identifier.issn	1741-427X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136302
dc.description.abstract	Copyright © 2018 Nisha Panth et al. This is an open access article distributed under the Creative Commons Attribution License Morus alba (white mulberry) is native to the northern part of Korea and popularly used as a traditional medicine due to its numerous health benefits against human's disease. However, the possibility that M. alba may also affect the cardiovascular system remains unexplored. This study sought to investigate the vascular protective effects of the root bark extract of M. alba (MAE). Vascular reactivity was performed in organ baths using isolated rat thoracic aorta, while platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMCs) were studied by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and wound healing assay, respectively. MAE evoked a concentration dependent vasorelaxation following endothelium-dependent pathway. However, vessel relaxations in response to MAE were markedly reduced after endothelium removal; treatment of endothelial nitric oxide synthase inhibitor, guanylyl cyclase inhibitor, and nonspecific potassium channel inhibitor, however, was not altered by cyclooxygenase inhibitor. Furthermore, MAE also significantly blunted contractile response to vasoconstrictor agent, phenylephrine. Taken together, the current evidence revealed that MAE is a potent endothelium-dependent vasodilator and this effect was involved in, at least in part, nitric oxide cyclic-guanosine monophosphate (NO-cGMP) pathway in combination with potassium (K + ) channel activation. Moreover, MAE inhibited proliferation and migration of VSMCs induced by PDGF. Therefore, MAE could be a promising candidate of natural medicine for preventing and controlling cardiovascular diseases linked with endothelial dysfunction.	en_US
dc.relation.ispartof	Evidence-based Complementary and Alternative Medicine	en_US
dc.relation.isbasedon	10.1155/2018/7905763	en_US
dc.subject.classification	Complementary & Alternative Medicine	en_US
dc.title	Vascular protection by ethanol extract of Morus alba root bark: Endothelium-dependent relaxation of rat aorta and decrease of smooth muscle cell migration and proliferation	en_US
dc.type	Journal Article
utslib.citation.volume	2018	en_US
utslib.for	1104 Complementary and Alternative Medicine	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	2018	en_US

Abstract:

Copyright © 2018 Nisha Panth et al. This is an open access article distributed under the Creative Commons Attribution License Morus alba (white mulberry) is native to the northern part of Korea and popularly used as a traditional medicine due to its numerous health benefits against human's disease. However, the possibility that M. alba may also affect the cardiovascular system remains unexplored. This study sought to investigate the vascular protective effects of the root bark extract of M. alba (MAE). Vascular reactivity was performed in organ baths using isolated rat thoracic aorta, while platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMCs) were studied by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and wound healing assay, respectively. MAE evoked a concentration dependent vasorelaxation following endothelium-dependent pathway. However, vessel relaxations in response to MAE were markedly reduced after endothelium removal; treatment of endothelial nitric oxide synthase inhibitor, guanylyl cyclase inhibitor, and nonspecific potassium channel inhibitor, however, was not altered by cyclooxygenase inhibitor. Furthermore, MAE also significantly blunted contractile response to vasoconstrictor agent, phenylephrine. Taken together, the current evidence revealed that MAE is a potent endothelium-dependent vasodilator and this effect was involved in, at least in part, nitric oxide cyclic-guanosine monophosphate (NO-cGMP) pathway in combination with potassium (K + ) channel activation. Moreover, MAE inhibited proliferation and migration of VSMCs induced by PDGF. Therefore, MAE could be a promising candidate of natural medicine for preventing and controlling cardiovascular diseases linked with endothelial dysfunction.

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http://hdl.handle.net/10453/136302