Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
Zhanghao, K
Chen, X
Liu, W
Li, M
Liu, Y
Wang, Y
Luo, S
Wang, X
Shan, C
Xie, H
Gao, J
Jin, D
Li, X
Zhang, Y
Dai, Q
Xi, P
- Publication Type:
- Journal Article
- Citation:
- Nature Communications, 2019, 10 (1)
- Issue Date:
- 2019-12-01
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author |
Zhanghao, K https://orcid.org/0000-0002-8331-6240 |
en_US |
dc.contributor.author | Chen, X | en_US |
dc.contributor.author |
Liu, W https://orcid.org/0000-0002-3003-1313 |
en_US |
dc.contributor.author | Li, M | en_US |
dc.contributor.author | Liu, Y | en_US |
dc.contributor.author | Wang, Y | en_US |
dc.contributor.author | Luo, S | en_US |
dc.contributor.author | Wang, X | en_US |
dc.contributor.author | Shan, C | en_US |
dc.contributor.author | Xie, H | en_US |
dc.contributor.author | Gao, J | en_US |
dc.contributor.author |
Jin, D https://orcid.org/0000-0003-1046-2666 |
en_US |
dc.contributor.author |
Li, X https://orcid.org/0000-0001-8677-9833 |
en_US |
dc.contributor.author | Zhang, Y | en_US |
dc.contributor.author | Dai, Q | en_US |
dc.contributor.author |
Xi, P https://orcid.org/0000-0001-6626-4840 |
en_US |
dc.date.available | 2019-09-20 | en_US |
dc.date.issued | 2019-12-01 | en_US |
dc.identifier.citation | Nature Communications, 2019, 10 (1) | en_US |
dc.identifier.uri | http://hdl.handle.net/10453/136577 | |
dc.description.abstract | © 2019, The Author(s). Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of bio-complexes. However, current techniques remain difficult to resolve the dipole assemblies on subcellular structures and their dynamics in living cells at super-resolution level. Here we report polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular hyperspace. We demonstrate the application of pSIM on a series of biological filamentous systems, such as cytoskeleton networks and λ-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. Further, pSIM reveals the side-by-side organization of the actin ring structures in the membrane-associated periodic skeleton of hippocampal neurons and images the dipole dynamics of green fluorescent protein-labeled microtubules in live U2OS cells. pSIM applies directly to a large variety of commercial and home-built SIM systems with various imaging modality. | en_US |
dc.relation.ispartof | Nature Communications | en_US |
dc.relation.isbasedon | 10.1038/s41467-019-12681-w | en_US |
dc.subject.mesh | Kidney | en_US |
dc.subject.mesh | Hippocampus | en_US |
dc.subject.mesh | Neurons | en_US |
dc.subject.mesh | Cell Line, Tumor | en_US |
dc.subject.mesh | Cytoskeleton | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Bacteriophage lambda | en_US |
dc.subject.mesh | Actins | en_US |
dc.subject.mesh | Myosins | en_US |
dc.subject.mesh | DNA | en_US |
dc.subject.mesh | Microscopy, Fluorescence | en_US |
dc.subject.mesh | Microscopy, Polarization | en_US |
dc.title | Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy | en_US |
dc.type | Journal Article | |
utslib.citation.volume | 1 | en_US |
utslib.citation.volume | 10 | en_US |
utslib.for | 0601 Biochemistry and Cell Biology | en_US |
utslib.for | 0605 Microbiology | en_US |
pubs.embargo.period | Not known | en_US |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Computer Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | /University of Technology Sydney/Strength - AAI - Advanced Analytics Institute Research Centre | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
utslib.copyright.status | open_access | |
pubs.issue | 1 | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 10 | en_US |
Abstract:
© 2019, The Author(s). Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of bio-complexes. However, current techniques remain difficult to resolve the dipole assemblies on subcellular structures and their dynamics in living cells at super-resolution level. Here we report polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular hyperspace. We demonstrate the application of pSIM on a series of biological filamentous systems, such as cytoskeleton networks and λ-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. Further, pSIM reveals the side-by-side organization of the actin ring structures in the membrane-associated periodic skeleton of hippocampal neurons and images the dipole dynamics of green fluorescent protein-labeled microtubules in live U2OS cells. pSIM applies directly to a large variety of commercial and home-built SIM systems with various imaging modality.
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