The role of FRIGIDA and FLOWERING LOCUS C genes in flowering time of Brassica rapa leafy vegetables

Takada, S; Akter, A; Itabashi, E; Nishida, N; Shea, DJ; Miyaji, N; Mehraj, H; Osabe, K; Shimizu, M; Takasaki-Yasuda, T; Kakizaki, T; Okazaki, K; Dennis, ES; Fujimoto, R

The role of FRIGIDA and FLOWERING LOCUS C genes in flowering time of Brassica rapa leafy vegetables

Takada, S

Akter, A Itabashi, E Nishida, N Shea, DJ

Miyaji, N Mehraj, H Osabe, K Shimizu, M Takasaki-Yasuda, T Kakizaki, T

Okazaki, K Dennis, ES

Fujimoto, R

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2019, 9 (1)
Issue Date:: 2019-12-01

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Field	Value	Language
dc.contributor.author	Takada, S https://orcid.org/0000-0003-4690-1739	en_US
dc.contributor.author	Akter, A	en_US
dc.contributor.author	Itabashi, E	en_US
dc.contributor.author	Nishida, N	en_US
dc.contributor.author	Shea, DJ https://orcid.org/0000-0001-8588-0462	en_US
dc.contributor.author	Miyaji, N	en_US
dc.contributor.author	Mehraj, H	en_US
dc.contributor.author	Osabe, K	en_US
dc.contributor.author	Shimizu, M	en_US
dc.contributor.author	Takasaki-Yasuda, T	en_US
dc.contributor.author	Kakizaki, T https://orcid.org/0000-0002-3636-6616	en_US
dc.contributor.author	Okazaki, K	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.contributor.author	Fujimoto, R https://orcid.org/0000-0003-4690-1739	en_US
dc.date.available	2019-09-03	en_US
dc.date.issued	2019-12-01	en_US
dc.identifier.citation	Scientific Reports, 2019, 9 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136578
dc.description.abstract	© 2019, The Author(s). There is a wide variation of flowering time among lines of Brassica rapa L. Most B. rapa leafy (Chinese cabbage etc.) or root (turnip) vegetables require prolonged cold exposure for flowering, known as vernalization. Premature bolting caused by low temperature leads to a reduction in the yield/quality of these B. rapa vegetables. Therefore, high bolting resistance is an important breeding trait, and understanding the molecular mechanism of vernalization is necessary to achieve this goal. In this study, we demonstrated that BrFRIb functions as an activator of BrFLC in B. rapa. We showed a positive correlation between the steady state expression levels of the sum of the BrFLC paralogs and the days to flowering after four weeks of cold treatment, suggesting that this is an indicator of the vernalization requirement. We indicate that BrFLCs are repressed by the accumulation of H3K27me3 and that the spreading of H3K27me3 promotes stable FLC repression. However, there was no clear relationship between the level of H3K27me3 in the BrFLC and the vernalization requirement. We also showed that if there was a high vernalization requirement, the rate of repression of BrFLC1 expression following prolonged cold treatments was lower.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/s41598-019-50122-2	en_US
dc.title	The role of FRIGIDA and FLOWERING LOCUS C genes in flowering time of Brassica rapa leafy vegetables	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	9	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

© 2019, The Author(s). There is a wide variation of flowering time among lines of Brassica rapa L. Most B. rapa leafy (Chinese cabbage etc.) or root (turnip) vegetables require prolonged cold exposure for flowering, known as vernalization. Premature bolting caused by low temperature leads to a reduction in the yield/quality of these B. rapa vegetables. Therefore, high bolting resistance is an important breeding trait, and understanding the molecular mechanism of vernalization is necessary to achieve this goal. In this study, we demonstrated that BrFRIb functions as an activator of BrFLC in B. rapa. We showed a positive correlation between the steady state expression levels of the sum of the BrFLC paralogs and the days to flowering after four weeks of cold treatment, suggesting that this is an indicator of the vernalization requirement. We indicate that BrFLCs are repressed by the accumulation of H3K27me3 and that the spreading of H3K27me3 promotes stable FLC repression. However, there was no clear relationship between the level of H3K27me3 in the BrFLC and the vernalization requirement. We also showed that if there was a high vernalization requirement, the rate of repression of BrFLC1 expression following prolonged cold treatments was lower.

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http://hdl.handle.net/10453/136578