Urban beaches are environmental hotspots for antibiotic resistance following rainfall

Carney, RL; Labbate, M; Siboni, N; Tagg, KA; Mitrovic, SM; Seymour, JR

Urban beaches are environmental hotspots for antibiotic resistance following rainfall

Carney, RL

Labbate, M

Siboni, N

Tagg, KA Mitrovic, SM

Seymour, JR

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Publication Type:: Journal Article
Citation:: Water Research, 2019, 167
Issue Date:: 2019-12-15

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Field	Value	Language
dc.contributor.author	Carney, RL https://orcid.org/0000-0001-8192-6507	en_US
dc.contributor.author	Labbate, M https://orcid.org/0000-0003-1428-3382	en_US
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949	en_US
dc.contributor.author	Tagg, KA	en_US
dc.contributor.author	Mitrovic, SM https://orcid.org/0000-0002-5528-2215	en_US
dc.contributor.author	Seymour, JR https://orcid.org/0000-0002-3745-6541	en_US
dc.date.available	2019-09-10	en_US
dc.date.issued	2019-12-15	en_US
dc.identifier.citation	Water Research, 2019, 167	en_US
dc.identifier.issn	0043-1354	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136650
dc.description.abstract	© 2019 Elsevier Ltd To reveal the occurrence and mechanisms for dispersal of antibiotic resistance (AbR) among the microbial assemblages inhabiting impacted coastal environments, we performed a weekly, two-year duration time-series study at two urban beaches between 2014 and 2016. We combined quantitative PCR and multiplex PCR/reverse line blot techniques to track patterns in the occurrence of 31 AbR genes, including genes that confer resistance to antibiotics that are critically important antimicrobials for human medicine. Patterns in the abundance of these genes were linked to specific microbial groups and environmental parameters by coupling qPCR and 16S rRNA amplicon sequencing data with network analysis. Up to 100-fold increases in the abundance of several AbR genes, including genes conferring resistance to quinolones, trimethoprim, sulfonamides, tetracycline, vancomycin and carbapenems, occurred following storm-water and modelled wet-weather sewer overflow events. The abundance of AbR genes strongly and significantly correlated with several potentially pathogenic bacterial OTUs regularly associated with wastewater infrastructure, such as Arcobacter, Acinetobacter, Aeromonas and Cloacibacterium. These high-resolution observations provide clear links between storm-water discharge and sewer overflow events and the occurrence of AbR in the coastal microbial assemblages inhabiting urban beaches, highlighting a direct mechanism for potentially significant AbR exposure risks to humans.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT130100218
dc.relation.ispartof	Water Research	en_US
dc.relation.isbasedon	10.1016/j.watres.2019.115081	en_US
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject.classification	Environmental Engineering	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Tetracycline	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Drug Resistance, Microbial	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Waste Water	en_US
dc.title	Urban beaches are environmental hotspots for antibiotic resistance following rainfall	en_US
dc.type	Journal Article
utslib.citation.volume	167	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2021-12-01T00:00:00+1100
pubs.publication-status	Published	en_US
pubs.volume	167	en_US

Abstract:

© 2019 Elsevier Ltd To reveal the occurrence and mechanisms for dispersal of antibiotic resistance (AbR) among the microbial assemblages inhabiting impacted coastal environments, we performed a weekly, two-year duration time-series study at two urban beaches between 2014 and 2016. We combined quantitative PCR and multiplex PCR/reverse line blot techniques to track patterns in the occurrence of 31 AbR genes, including genes that confer resistance to antibiotics that are critically important antimicrobials for human medicine. Patterns in the abundance of these genes were linked to specific microbial groups and environmental parameters by coupling qPCR and 16S rRNA amplicon sequencing data with network analysis. Up to 100-fold increases in the abundance of several AbR genes, including genes conferring resistance to quinolones, trimethoprim, sulfonamides, tetracycline, vancomycin and carbapenems, occurred following storm-water and modelled wet-weather sewer overflow events. The abundance of AbR genes strongly and significantly correlated with several potentially pathogenic bacterial OTUs regularly associated with wastewater infrastructure, such as Arcobacter, Acinetobacter, Aeromonas and Cloacibacterium. These high-resolution observations provide clear links between storm-water discharge and sewer overflow events and the occurrence of AbR in the coastal microbial assemblages inhabiting urban beaches, highlighting a direct mechanism for potentially significant AbR exposure risks to humans.

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http://hdl.handle.net/10453/136650