Quantitative Nondestructive Assessment of Paenibacillus larvae in Apis mellifera Hives

Lyall, D; Hansbro, P; Horvat, J; Stanwell, P

Quantitative Nondestructive Assessment of Paenibacillus larvae in Apis mellifera Hives

Lyall, D Hansbro, P

Horvat, J Stanwell, P

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Publication Type:: Conference Proceeding
Citation:: Advances in Intelligent Systems and Computing, 2020, 1026 pp. 579 - 583
Issue Date:: 2020-01-01

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Field	Value	Language
dc.contributor.author	Lyall, D	en_US
dc.contributor.author	Hansbro, P https://orcid.org/0000-0002-4741-3035	en_US
dc.contributor.author	Horvat, J	en_US
dc.contributor.author	Stanwell, P	en_US
dc.date.issued	2020-01-01	en_US
dc.identifier.citation	Advances in Intelligent Systems and Computing, 2020, 1026 pp. 579 - 583	en_US
dc.identifier.isbn	9783030279271	en_US
dc.identifier.issn	2194-5357	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136754
dc.description.abstract	© Springer Nature Switzerland AG 2020. Paenibacillus larvae is a Gram-positive spore forming bacterium and is the causative agent of American Foulbrood (AFB), a highly contagious infectious disease of Apis Mellifera (the European honey bee), which results in hive death and significant economic losses within the apiary industry world-wide. AFB is typically diagnosed by visual identification which is labour intensive and highly operator dependent. In the absence of a low cost, rapid and reliable industry surveillance tool targeting AFB, the disease has spread around the world. Real-time polymerase chain reaction (rtPCR) is a potential AFB surveillance tool with a demonstrated high sensitivity for the detection and quantification of Paenibacillus larvae infections in Apis Mellifera hives. Current techniques result in the disturbance of the internal hive microclimate by opening and deconstructing the hive. Our research aims to develop a new, non-invasive, rtPCR technique for the detection of AFB in beehives without the need to open the hive.	en_US
dc.relation.ispartof	Advances in Intelligent Systems and Computing	en_US
dc.relation.isbasedon	10.1007/978-3-030-27928-8_88	en_US
dc.title	Quantitative Nondestructive Assessment of Paenibacillus larvae in Apis mellifera Hives	en_US
dc.type	Conference Proceeding
utslib.citation.volume	1026	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	1026	en_US

Abstract:

© Springer Nature Switzerland AG 2020. Paenibacillus larvae is a Gram-positive spore forming bacterium and is the causative agent of American Foulbrood (AFB), a highly contagious infectious disease of Apis Mellifera (the European honey bee), which results in hive death and significant economic losses within the apiary industry world-wide. AFB is typically diagnosed by visual identification which is labour intensive and highly operator dependent. In the absence of a low cost, rapid and reliable industry surveillance tool targeting AFB, the disease has spread around the world. Real-time polymerase chain reaction (rtPCR) is a potential AFB surveillance tool with a demonstrated high sensitivity for the detection and quantification of Paenibacillus larvae infections in Apis Mellifera hives. Current techniques result in the disturbance of the internal hive microclimate by opening and deconstructing the hive. Our research aims to develop a new, non-invasive, rtPCR technique for the detection of AFB in beehives without the need to open the hive.

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http://hdl.handle.net/10453/136754