The histone modification H3 lysine 27 tri-methylation has conserved gene regulatory roles in the triplicated genome of Brassica rapa L

Akter, A; Takahashi, S; Deng, W; Shea, DJ; Itabashi, E; Shimizu, M; Miyaji, N; Osabe, K; Nishida, N; Suzuki, Y; Helliwell, CA; Seki, M; Peacock, WJ; Dennis, ES; Fujimoto, R

The histone modification H3 lysine 27 tri-methylation has conserved gene regulatory roles in the triplicated genome of Brassica rapa L

Akter, A Takahashi, S Deng, W Shea, DJ Itabashi, E Shimizu, M Miyaji, N Osabe, K Nishida, N Suzuki, Y Helliwell, CA Seki, M Peacock, WJ

Dennis, ES

Fujimoto, R

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Publication Type:: Journal Article
Citation:: DNA research : an international journal for rapid publication of reports on genes and genomes, 2019, 26 (5), pp. 433 - 443
Issue Date:: 2019-10-01

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Field	Value	Language
dc.contributor.author	Akter, A	en_US
dc.contributor.author	Takahashi, S	en_US
dc.contributor.author	Deng, W	en_US
dc.contributor.author	Shea, DJ	en_US
dc.contributor.author	Itabashi, E	en_US
dc.contributor.author	Shimizu, M	en_US
dc.contributor.author	Miyaji, N	en_US
dc.contributor.author	Osabe, K	en_US
dc.contributor.author	Nishida, N	en_US
dc.contributor.author	Suzuki, Y	en_US
dc.contributor.author	Helliwell, CA	en_US
dc.contributor.author	Seki, M	en_US
dc.contributor.author	Peacock, WJ https://orcid.org/0000-0001-7314-9074	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.contributor.author	Fujimoto, R	en_US
dc.date.available	2019-08-30	en_US
dc.date.issued	2019-10-01	en_US
dc.identifier.citation	DNA research : an international journal for rapid publication of reports on genes and genomes, 2019, 26 (5), pp. 433 - 443	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136853
dc.description.abstract	© The Author(s) 2019. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. Brassica rapa L. is an important vegetable and oilseed crop. We investigated the distribution of the histone mark tri-methylation of H3K27 (H3K27me3) in B. rapa and its role in the control of gene expression at two stages of development (2-day cotyledons and 14-day leaves) and among paralogs in the triplicated genome. H3K27me3 has a similar distribution in two inbred lines, while there was variation of H3K27me3 sites between tissues. Sites that are specific to 2-day cotyledons have increased transcriptional activity, and low levels of H3K27me3 in the gene body region. In 14-day leaves, levels of H3K27me3 were associated with decreased gene expression. In the triplicated genome, H3K27me3 is associated with paralogs that have tissue-specific expression. Even though B. rapa and Arabidopsis thaliana are not closely related within the Brassicaceae, there is conservation of H3K27me3-marked sites in the two species. Both B. rapa and A. thaliana require vernalization for floral initiation with FLC being the major controlling locus. In all four BrFLC paralogs, low-temperature treatment increases H3K27me3 at the proximal nucleation site reducing BrFLC expression. Following return to normal temperature growth conditions, H3K27me3 spreads along all four BrFLC paralogs providing stable repression of the gene.	en_US
dc.relation.ispartof	DNA research : an international journal for rapid publication of reports on genes and genomes	en_US
dc.relation.isbasedon	10.1093/dnares/dsz021	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Brassica rapa	en_US
dc.subject.mesh	Histones	en_US
dc.subject.mesh	Epigenesis, Genetic	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Protein Processing, Post-Translational	en_US
dc.subject.mesh	Histone Code	en_US
dc.subject.mesh	Methylation	en_US
dc.subject.mesh	Polyploidy	en_US
dc.title	The histone modification H3 lysine 27 tri-methylation has conserved gene regulatory roles in the triplicated genome of Brassica rapa L	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	26	en_US
utslib.for	0703 Crop and Pasture Production	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	26	en_US

Abstract:

© The Author(s) 2019. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. Brassica rapa L. is an important vegetable and oilseed crop. We investigated the distribution of the histone mark tri-methylation of H3K27 (H3K27me3) in B. rapa and its role in the control of gene expression at two stages of development (2-day cotyledons and 14-day leaves) and among paralogs in the triplicated genome. H3K27me3 has a similar distribution in two inbred lines, while there was variation of H3K27me3 sites between tissues. Sites that are specific to 2-day cotyledons have increased transcriptional activity, and low levels of H3K27me3 in the gene body region. In 14-day leaves, levels of H3K27me3 were associated with decreased gene expression. In the triplicated genome, H3K27me3 is associated with paralogs that have tissue-specific expression. Even though B. rapa and Arabidopsis thaliana are not closely related within the Brassicaceae, there is conservation of H3K27me3-marked sites in the two species. Both B. rapa and A. thaliana require vernalization for floral initiation with FLC being the major controlling locus. In all four BrFLC paralogs, low-temperature treatment increases H3K27me3 at the proximal nucleation site reducing BrFLC expression. Following return to normal temperature growth conditions, H3K27me3 spreads along all four BrFLC paralogs providing stable repression of the gene.

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http://hdl.handle.net/10453/136853