Single, double and triple mutants of Salmonella enterica serovar Typhimurium degP (htrA), degQ (hhoA) and degS (hhoB) have diverse phenotypes on exposure to elevated temperature and their growth in vivo is attenuated to different extents.

Mo, E; Peters, SE; Willers, C; Maskell, DJ; Charles, IG

Single, double and triple mutants of Salmonella enterica serovar Typhimurium degP (htrA), degQ (hhoA) and degS (hhoB) have diverse phenotypes on exposure to elevated temperature and their growth in vivo is attenuated to different extents.

Mo, E Peters, SE Willers, C Maskell, DJ Charles, IG

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Publication Type:: Journal Article
Citation:: Microb Pathog, 2006, 41 (4-5), pp. 174 - 182
Issue Date:: 2006-10

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Field	Value	Language
dc.contributor.author	Mo, E	en_US
dc.contributor.author	Peters, SE	en_US
dc.contributor.author	Willers, C	en_US
dc.contributor.author	Maskell, DJ	en_US
dc.contributor.author	Charles, IG	en_US
dc.date.available	2006-07-11	en_US
dc.date.issued	2006-10	en_US
dc.identifier.citation	Microb Pathog, 2006, 41 (4-5), pp. 174 - 182	en_US
dc.identifier.issn	0882-4010	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13738
dc.description.abstract	DegP (HtrA) is a well-studied protease involved in survival of bacteria under stress conditions in vitro and in vivo. There are two paralogues of DegP in the Salmonella enterica serovar Typhimurium genome, DegQ and DegS. In order to understand more about the biological significance of this gene family, a series of deg-deletion mutants was generated in S. Typhimurium strain SL3261 by allelic replacement. At elevated temperature in vitro, the viability of degP and degS mutants was reduced when compared with the parent strain whereas the viability of a degQ mutant was not significantly affected. The viability of a double degP-degS mutant at elevated temperature was severely decreased when compared with the respective single mutants or, interestingly, with a triple degP-degQ-degS mutant. All the deg deletions were transduced into the mouse-virulent strain SL1344 and the resultant mutants were injected intravenously into BALB/c mice to test virulence. degP and degS single mutants and all combinations of double and triple mutants were attenuated to different degrees, whereas the single degQ mutant was as virulent as the wild-type strain. Thus, within this gene family, degP and degS appear important for survival at elevated temperature and are necessary for full virulence, whereas a single degQ deletion appears to have no clear role in survival and growth at elevated temperature or in mice.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Microb Pathog	en_US
dc.relation.isbasedon	10.1016/j.micpath.2006.07.004	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Liver	en_US
dc.subject.mesh	Spleen	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred BALB C	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Salmonella typhimurium	en_US
dc.subject.mesh	Salmonella Infections	en_US
dc.subject.mesh	Serine Endopeptidases	en_US
dc.subject.mesh	Periplasmic Proteins	en_US
dc.subject.mesh	Heat-Shock Proteins	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Biological Assay	en_US
dc.subject.mesh	Mutagenesis, Insertional	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Heat-Shock Response	en_US
dc.subject.mesh	Phenotype	en_US
dc.title	Single, double and triple mutants of Salmonella enterica serovar Typhimurium degP (htrA), degQ (hhoA) and degS (hhoB) have diverse phenotypes on exposure to elevated temperature and their growth in vivo is attenuated to different extents.	en_US
dc.type	Journal Article
utslib.citation.volume	4-5	en_US
utslib.citation.volume	41	en_US
utslib.location.activity	England	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1107 Immunology	en_US
utslib.for	1108 Medical Microbiology	en_US
dc.location.activity	ISI:000241282500007	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	4-5	en_US
pubs.publication-status	Published	en_US
pubs.volume	41	en_US

Abstract:

DegP (HtrA) is a well-studied protease involved in survival of bacteria under stress conditions in vitro and in vivo. There are two paralogues of DegP in the Salmonella enterica serovar Typhimurium genome, DegQ and DegS. In order to understand more about the biological significance of this gene family, a series of deg-deletion mutants was generated in S. Typhimurium strain SL3261 by allelic replacement. At elevated temperature in vitro, the viability of degP and degS mutants was reduced when compared with the parent strain whereas the viability of a degQ mutant was not significantly affected. The viability of a double degP-degS mutant at elevated temperature was severely decreased when compared with the respective single mutants or, interestingly, with a triple degP-degQ-degS mutant. All the deg deletions were transduced into the mouse-virulent strain SL1344 and the resultant mutants were injected intravenously into BALB/c mice to test virulence. degP and degS single mutants and all combinations of double and triple mutants were attenuated to different degrees, whereas the single degQ mutant was as virulent as the wild-type strain. Thus, within this gene family, degP and degS appear important for survival at elevated temperature and are necessary for full virulence, whereas a single degQ deletion appears to have no clear role in survival and growth at elevated temperature or in mice.

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http://hdl.handle.net/10453/13738