Anti-HIV-1 protease activity of the crude extracts and isolated compounds from Auricularia polytricha

Sillapachaiyaporn, C; Nilkhet, S; Ung, AT; Chuchawankul, S

Anti-HIV-1 protease activity of the crude extracts and isolated compounds from Auricularia polytricha

Sillapachaiyaporn, C Nilkhet, S Ung, AT

Chuchawankul, S

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Publication Type:: Journal Article
Citation:: BMC Complementary and Alternative Medicine, 2019, 19 (1)
Issue Date:: 2019-12-05

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Field	Value	Language
dc.contributor.author	Sillapachaiyaporn, C	en_US
dc.contributor.author	Nilkhet, S	en_US
dc.contributor.author	Ung, AT https://orcid.org/0000-0002-5665-0702	en_US
dc.contributor.author	Chuchawankul, S https://orcid.org/0000-0002-7627-0408	en_US
dc.date.available	2019-11-21	en_US
dc.date.issued	2019-12-05	en_US
dc.identifier.citation	BMC Complementary and Alternative Medicine, 2019, 19 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/137835
dc.description.abstract	© 2019 The Author(s). Background: Acquired immunodeficiency syndrome (AIDS) is caused by the Human immunodeficiency virus type-1 (HIV-1). HIV-1 protease (HIV-1 PR) is an essential enzyme for the HIV replication, and therefore, it is an important target for antiretroviral drugs development, particularly from natural products. Auricularia polytricha (AP) is an edible mushroom with several important therapeutic properties. These properties will be investigated as HIV-1 PR inhibitors. Methods: The sequential hexane (APH), ethanol (APE) and water (APW) extracts from AP were screened for inhibitory activity against HIV-1 PR. The extract that consistently showed the strong HIV-1 PR inhibition was further investigated for its phytochemical constituents. The compounds were purified by column chromatography. The isolated compounds were structurally elucidated using 1D and 2D NMR, HRMS, FTIR, and GC/MS techniques. Each compound was screened against HIV-1 PR to determine its inhibitory activity and to provide an explanation for the activity found in the extract. Results: Hexane crude extract of AP (APH) exhibited significant inhibition on HIV-1 PR activity. Four major compounds isolated from APH fraction were identified to be two triacylglycerols, linoleic acid and ergosterol. Moreover, all four compounds showed significant inhibition of HIV-1 PR activity. Conclusion: The findings from this study suggest that AP is a good source of fatty esters, fatty acids and ergosterol. These natural products exhibit anti-HIV-1 properties by blocking HIV-1 PR. These important biological results warrant further development of AP as an alternative antiretroviral drug.	en_US
dc.relation.ispartof	BMC Complementary and Alternative Medicine	en_US
dc.relation.isbasedon	10.1186/s12906-019-2766-3	en_US
dc.subject.classification	Complementary & Alternative Medicine	en_US
dc.subject.mesh	3T3-L1 Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	HIV-1	en_US
dc.subject.mesh	Agaricales	en_US
dc.subject.mesh	Ergosterol	en_US
dc.subject.mesh	HIV Protease	en_US
dc.subject.mesh	Linoleic Acid	en_US
dc.subject.mesh	Triglycerides	en_US
dc.subject.mesh	Biological Products	en_US
dc.subject.mesh	HIV Protease Inhibitors	en_US
dc.subject.mesh	Cell Survival	en_US
dc.title	Anti-HIV-1 protease activity of the crude extracts and isolated compounds from Auricularia polytricha	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	19	en_US
utslib.for	1104 Complementary and Alternative Medicine	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	19	en_US

Abstract:

© 2019 The Author(s). Background: Acquired immunodeficiency syndrome (AIDS) is caused by the Human immunodeficiency virus type-1 (HIV-1). HIV-1 protease (HIV-1 PR) is an essential enzyme for the HIV replication, and therefore, it is an important target for antiretroviral drugs development, particularly from natural products. Auricularia polytricha (AP) is an edible mushroom with several important therapeutic properties. These properties will be investigated as HIV-1 PR inhibitors. Methods: The sequential hexane (APH), ethanol (APE) and water (APW) extracts from AP were screened for inhibitory activity against HIV-1 PR. The extract that consistently showed the strong HIV-1 PR inhibition was further investigated for its phytochemical constituents. The compounds were purified by column chromatography. The isolated compounds were structurally elucidated using 1D and 2D NMR, HRMS, FTIR, and GC/MS techniques. Each compound was screened against HIV-1 PR to determine its inhibitory activity and to provide an explanation for the activity found in the extract. Results: Hexane crude extract of AP (APH) exhibited significant inhibition on HIV-1 PR activity. Four major compounds isolated from APH fraction were identified to be two triacylglycerols, linoleic acid and ergosterol. Moreover, all four compounds showed significant inhibition of HIV-1 PR activity. Conclusion: The findings from this study suggest that AP is a good source of fatty esters, fatty acids and ergosterol. These natural products exhibit anti-HIV-1 properties by blocking HIV-1 PR. These important biological results warrant further development of AP as an alternative antiretroviral drug.

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http://hdl.handle.net/10453/137835