Hackflex: low cost Illumina sequencing library construction for high sample counts

Gaio, D; To, J; Liu, M; Monahan, L; Anantanawat, K; Darling, A

Hackflex: low cost Illumina sequencing library construction for high sample counts

Gaio, D

To, J

Liu, M Monahan, L

Anantanawat, K

Darling, A

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Publication Type:: Journal Article
Citation:: 2019
Issue Date:: 2019-09-23

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Field	Value	Language
dc.contributor.author	Gaio, D https://orcid.org/0000-0002-7695-3145	en_US
dc.contributor.author	To, J https://orcid.org/0000-0003-3482-4369	en_US
dc.contributor.author	Liu, M	en_US
dc.contributor.author	Monahan, L https://orcid.org/0000-0003-2933-9000	en_US
dc.contributor.author	Anantanawat, K https://orcid.org/0000-0002-7862-0969	en_US
dc.contributor.author	Darling, A https://orcid.org/0000-0003-2397-7925	en_US
dc.date.issued	2019-09-23	en_US
dc.identifier.citation	2019	en_US
dc.identifier.uri	http://hdl.handle.net/10453/137849
dc.description.abstract	ABSTRACT We developed Hackflex, a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 11 times more libraries for high-throughput Illumina sequencing to be generated at a fixed cost. We call this new method Hackflex. Quality of library preparation was tested by constructing libraries from E. coli MG1655 genomic DNA using either Hackflex, standard Nextera Flex or a variation of standard Nextera Flex in which the bead-linked transposase is diluted prior to use. We demonstrated that Hackflex can produce high quality libraries and yields a highly uniform coverage, equivalent to the standard Nextera Flex kit. Using Hackflex, we were able to achieve a per sample reagent cost of library prep of A$8.66, which is 8.23 times lower than the Standard Nextera Flex protocol at advertised retail price. An additional simple modification to the protocol enables a further price reduction of up to 11 fold or about A$6.50/sample. This method will allow researchers to construct more libraries within a given budget, thereby yielding more data and facilitating research programs where sequencing large numbers of libraries is beneficial.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LP150100912
dc.relation.isbasedon	10.1101/779215	en_US
dc.title	Hackflex: low cost Illumina sequencing library construction for high sample counts	en_US
dc.type	Journal Article
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US

Abstract:

ABSTRACT We developed Hackflex, a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 11 times more libraries for high-throughput Illumina sequencing to be generated at a fixed cost. We call this new method Hackflex. Quality of library preparation was tested by constructing libraries from E. coli MG1655 genomic DNA using either Hackflex, standard Nextera Flex or a variation of standard Nextera Flex in which the bead-linked transposase is diluted prior to use. We demonstrated that Hackflex can produce high quality libraries and yields a highly uniform coverage, equivalent to the standard Nextera Flex kit. Using Hackflex, we were able to achieve a per sample reagent cost of library prep of A$8.66, which is 8.23 times lower than the Standard Nextera Flex protocol at advertised retail price. An additional simple modification to the protocol enables a further price reduction of up to 11 fold or about A$6.50/sample. This method will allow researchers to construct more libraries within a given budget, thereby yielding more data and facilitating research programs where sequencing large numbers of libraries is beneficial.

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http://hdl.handle.net/10453/137849