Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking cystic fibrosis lung sputum

Fung, C; Naughton, S; Turnbull, L; Tingpej, P; Rose, B; Arthur, J; Hu, H; Harmer, C; Harbour, C; Hassett, DJ; Whitchurch, CB; Manos, J

Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking cystic fibrosis lung sputum

Fung, C Naughton, S Turnbull, L

Tingpej, P Rose, B Arthur, J Hu, H Harmer, C Harbour, C Hassett, DJ Whitchurch, CB

Manos, J

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Publication Type:: Journal Article
Citation:: Journal of Medical Microbiology, 2010, 59 (9), pp. 1089 - 1100
Issue Date:: 2010-09-01

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Field	Value	Language
dc.contributor.author	Fung, C	en_US
dc.contributor.author	Naughton, S	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Tingpej, P	en_US
dc.contributor.author	Rose, B	en_US
dc.contributor.author	Arthur, J	en_US
dc.contributor.author	Hu, H	en_US
dc.contributor.author	Harmer, C	en_US
dc.contributor.author	Harbour, C	en_US
dc.contributor.author	Hassett, DJ	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Manos, J	en_US
dc.date.issued	2010-09-01	en_US
dc.identifier.citation	Journal of Medical Microbiology, 2010, 59 (9), pp. 1089 - 1100	en_US
dc.identifier.issn	0022-2615	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13815
dc.description.abstract	Pseudomonas aeruginosa airway infection is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. Various in vitro models have been developed to study P. aeruginosa pathobiology in the CF lung. In this study we produced a modified artificial-sputum medium (ASMDM) more closely resembling CF sputum than previous models, and extended previous work by using strain PAO1 arrays to examine the global transcription profiles of P. aeruginosa strain UCBPP-PA14 under early exponential-phase and stationary-phase growth. In early exponential phase, 38/39 nutrition-related genes were upregulated in line with data from previous in vitro models using UCBPP-PA14. Additionally, 23 type III secretion system (T3SS) genes, several anaerobic respiration genes and 24 quorum-sensing (QS)-related genes were upregulated in ASMDM, suggesting enhanced virulence factor expression and priming for anaerobic growth and biofilm formation. Under stationary phase growth in ASMDM, macroscopic clumps resembling microcolonies were evident in UCBPP-PA14 and CF strains, and over 40 potentially important genes were differentially expressed relative to stationary-phase growth in Luria broth. Most notably, QS-related and T3SS genes were downregulated in ASMDM, and iron-acquisition and assimilatory nitrate reductase genes were upregulated, simulating the iron-depleted, microaerophilic/anaerobic environment of CF sputum. ASMDM thus appears to be highly suitable for gene expression studies of P. aeruginosa in CF. © 2010 SGM Printed in Great Britain.	en_US
dc.relation.ispartof	Journal of Medical Microbiology	en_US
dc.relation.isbasedon	10.1099/jmm.0.019984-0	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Sputum	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Cystic Fibrosis	en_US
dc.subject.mesh	Mucins	en_US
dc.subject.mesh	Culture Media	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.title	Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking cystic fibrosis lung sputum	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	59	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	59	en_US

Abstract:

Pseudomonas aeruginosa airway infection is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. Various in vitro models have been developed to study P. aeruginosa pathobiology in the CF lung. In this study we produced a modified artificial-sputum medium (ASMDM) more closely resembling CF sputum than previous models, and extended previous work by using strain PAO1 arrays to examine the global transcription profiles of P. aeruginosa strain UCBPP-PA14 under early exponential-phase and stationary-phase growth. In early exponential phase, 38/39 nutrition-related genes were upregulated in line with data from previous in vitro models using UCBPP-PA14. Additionally, 23 type III secretion system (T3SS) genes, several anaerobic respiration genes and 24 quorum-sensing (QS)-related genes were upregulated in ASMDM, suggesting enhanced virulence factor expression and priming for anaerobic growth and biofilm formation. Under stationary phase growth in ASMDM, macroscopic clumps resembling microcolonies were evident in UCBPP-PA14 and CF strains, and over 40 potentially important genes were differentially expressed relative to stationary-phase growth in Luria broth. Most notably, QS-related and T3SS genes were downregulated in ASMDM, and iron-acquisition and assimilatory nitrate reductase genes were upregulated, simulating the iron-depleted, microaerophilic/anaerobic environment of CF sputum. ASMDM thus appears to be highly suitable for gene expression studies of P. aeruginosa in CF. © 2010 SGM Printed in Great Britain.

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http://hdl.handle.net/10453/13815