Eriobotrya japonica leaf extract attenuates airway inflammation in ovalbumin-induced mice model of asthma.

Kim, T-M; Paudel, KR; Kim, D-W

Eriobotrya japonica leaf extract attenuates airway inflammation in ovalbumin-induced mice model of asthma.

Kim, T-M Paudel, KR

Kim, D-W

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Publication Type:: Journal Article
Citation:: J Ethnopharmacol, 2019, 253 pp. 112082 - ?
Issue Date:: 2019-07-13

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Field	Value	Language
dc.contributor.author	Kim, T-M	en_US
dc.contributor.author	Paudel, KR https://orcid.org/0000-0002-3591-2080	en_US
dc.contributor.author	Kim, D-W	en_US
dc.date.available	2019-07-11	en_US
dc.date.issued	2019-07-13	en_US
dc.identifier.citation	J Ethnopharmacol, 2019, 253 pp. 112082 - ?	en_US
dc.identifier.uri	http://hdl.handle.net/10453/138436
dc.description.abstract	ETHNOPHARMACOLOGICAL RELEVANCE: Eriobotrya japonica leaves has a very long history of medicinal use as an anti-inflammatory and antitussive agent for bronchial inflammation. AIM OF THE STUDY: The aim of this study was to evaluate the anti-inflammatory activities of Eriobotrya japonica (EJ) leaf water extract in an ovalbumin (OVA)-induced murine asthma model and human tracheal smooth muscle cell (HTSMC). MATERIALS AND METHODS: Mice were sensitized by intra peritoneal OVA and challenged with nebulized OVA. EJ extract was administered orally at various dose. Bronchoalveolar lavage fluid (BALF) was quantified for nitric oxide (NO), eosinophil peroxidase (EPO), interleukin (IL)-4, IL-13 level and immunoglobulin (Ig) E was quantified in serum. Lung tissue sections were stained with hematoxylin and eosin for assessment of inflammatory cell infiltration whereas mucus production and goblet cell hyperplasia were studied by periodic acid schiff staining. Western blot was done for analysis of pERK1/2 expression and NFκB translocation in HTSMC whereas iNOS and COX-2 expression in RAW264.7 cell. RESULTS: EJ significantly reduced the levels of BALF's NO, EPO, MMPs, IL-4, IL-13, and serum IgE. It also decreases inflammatory cell infiltration and mucus production. EJ also attenuated the proliferation of HTSMC, inhibits overexpression of ERK 1/2 and translocation of NFκB in HTSMC as well as iNOS and COX-2 expression in RAW 264.7 cell. CONCLUSION: Present study suggest that, EJ effectively protects against allergic airway inflammation thus possessing potential therapeutic option for allergic asthma management.	en_US
dc.language	eng	en_US
dc.relation.ispartof	J Ethnopharmacol	en_US
dc.relation.isbasedon	10.1016/j.jep.2019.112082	en_US
dc.subject.classification	Medicinal & Biomolecular Chemistry	en_US
dc.title	Eriobotrya japonica leaf extract attenuates airway inflammation in ovalbumin-induced mice model of asthma.	en_US
dc.type	Journal Article
utslib.citation.volume	253	en_US
utslib.location.activity	Ireland	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	1104 Complementary and Alternative Medicine	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.publication-status	Published online	en_US
pubs.volume	253	en_US

Abstract:

ETHNOPHARMACOLOGICAL RELEVANCE: Eriobotrya japonica leaves has a very long history of medicinal use as an anti-inflammatory and antitussive agent for bronchial inflammation. AIM OF THE STUDY: The aim of this study was to evaluate the anti-inflammatory activities of Eriobotrya japonica (EJ) leaf water extract in an ovalbumin (OVA)-induced murine asthma model and human tracheal smooth muscle cell (HTSMC). MATERIALS AND METHODS: Mice were sensitized by intra peritoneal OVA and challenged with nebulized OVA. EJ extract was administered orally at various dose. Bronchoalveolar lavage fluid (BALF) was quantified for nitric oxide (NO), eosinophil peroxidase (EPO), interleukin (IL)-4, IL-13 level and immunoglobulin (Ig) E was quantified in serum. Lung tissue sections were stained with hematoxylin and eosin for assessment of inflammatory cell infiltration whereas mucus production and goblet cell hyperplasia were studied by periodic acid schiff staining. Western blot was done for analysis of pERK1/2 expression and NFκB translocation in HTSMC whereas iNOS and COX-2 expression in RAW264.7 cell. RESULTS: EJ significantly reduced the levels of BALF's NO, EPO, MMPs, IL-4, IL-13, and serum IgE. It also decreases inflammatory cell infiltration and mucus production. EJ also attenuated the proliferation of HTSMC, inhibits overexpression of ERK 1/2 and translocation of NFκB in HTSMC as well as iNOS and COX-2 expression in RAW 264.7 cell. CONCLUSION: Present study suggest that, EJ effectively protects against allergic airway inflammation thus possessing potential therapeutic option for allergic asthma management.

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http://hdl.handle.net/10453/138436