Dual RNA-Seq of Chlamydia and Host Cells

Marsh, JW; Hayward, RJ; Shetty, A; Mahurkar, A; Humphrys, MS; Myers, GSA

Dual RNA-Seq of Chlamydia and Host Cells

Marsh, JW

Hayward, RJ

Shetty, A Mahurkar, A Humphrys, MS Myers, GSA

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Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2019, 2042 pp. 123 - 135
Issue Date:: 2019-01-01

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Field	Value	Language
dc.contributor.author	Marsh, JW https://orcid.org/0000-0003-4681-1012	en_US
dc.contributor.author	Hayward, RJ https://orcid.org/0000-0002-6300-3271	en_US
dc.contributor.author	Shetty, A	en_US
dc.contributor.author	Mahurkar, A	en_US
dc.contributor.author	Humphrys, MS	en_US
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.date.issued	2019-01-01	en_US
dc.identifier.citation	Methods in Molecular Biology, 2019, 2042 pp. 123 - 135	en_US
dc.identifier.uri	http://hdl.handle.net/10453/138837
dc.description.abstract	© Springer Science+Business Media, LLC, part of Springer Nature 2019. During the infection of a host cell by a bacterial pathogen, a cascading series of gene expression changes occurs as each organism manipulates or responds to the other via defense or survival strategies. Unraveling this complex interplay is key for our understanding of bacterial virulence and host response pathways for the development of novel therapeutics. Dual RNA sequencing (dual RNA-Seq) has recently been developed to simultaneously capture host and bacterial transcriptomes from an infected cell. Leveraging the sensitivity and resolution allowed by RNA-seq, dual RNA-Seq can be applied to any bacteria–eukaryotic host interaction. We pioneered dual RNA-Seq to simultaneously capture Chlamydia and host expression profiles during an in vitro infection as proof of principle. Here we provide a detailed laboratory protocol and bioinformatics analysis guidelines for dual RNA-seq experiments focusing on Chlamydia as the organism of interest.	en_US
dc.relation.ispartof	Methods in Molecular Biology	en_US
dc.relation.isbasedon	10.1007/978-1-4939-9694-0_9	en_US
dc.subject.classification	Developmental Biology	en_US
dc.title	Dual RNA-Seq of Chlamydia and Host Cells	en_US
dc.type	Chapter
utslib.citation.volume	2042	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	2042	en_US

Abstract:

© Springer Science+Business Media, LLC, part of Springer Nature 2019. During the infection of a host cell by a bacterial pathogen, a cascading series of gene expression changes occurs as each organism manipulates or responds to the other via defense or survival strategies. Unraveling this complex interplay is key for our understanding of bacterial virulence and host response pathways for the development of novel therapeutics. Dual RNA sequencing (dual RNA-Seq) has recently been developed to simultaneously capture host and bacterial transcriptomes from an infected cell. Leveraging the sensitivity and resolution allowed by RNA-seq, dual RNA-Seq can be applied to any bacteria–eukaryotic host interaction. We pioneered dual RNA-Seq to simultaneously capture Chlamydia and host expression profiles during an in vitro infection as proof of principle. Here we provide a detailed laboratory protocol and bioinformatics analysis guidelines for dual RNA-seq experiments focusing on Chlamydia as the organism of interest.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/138837