Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species

Verma, A; Kohli, GS; Harwood, DT; Ralph, PJ; Murray, SA

Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species

Verma, A

Kohli, GS

Harwood, DT Ralph, PJ

Murray, SA

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Publication Type:: Journal Article
Citation:: Environmental Microbiology, 2019, 21 (11), pp. 4196 - 4211
Issue Date:: 2019-11-01

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Field	Value	Language
dc.contributor.author	Verma, A https://orcid.org/0000-0003-0119-9915	en_US
dc.contributor.author	Kohli, GS https://orcid.org/0000-0002-4578-2355	en_US
dc.contributor.author	Harwood, DT	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Murray, SA https://orcid.org/0000-0001-7096-1307	en_US
dc.date.available	2019-08-12	en_US
dc.date.issued	2019-11-01	en_US
dc.identifier.citation	Environmental Microbiology, 2019, 21 (11), pp. 4196 - 4211	en_US
dc.identifier.issn	1462-2912	en_US
dc.identifier.uri	http://hdl.handle.net/10453/139046
dc.description.abstract	© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd. In marine ecosystems, dinoflagellates can become highly abundant and even dominant at times, despite their comparatively slow growth. Their ecological success may be related to their production of complex toxic polyketide compounds. Ostreopsis species produce potent palytoxin-like compounds (PLTX), which are associated with human skin and eye irritations, and illnesses through the consumption of contaminated seafood. To investigate the genetic basis of PLTX-like compounds, we sequenced and annotated transcriptomes from two PLTX-producing Ostreopsis species; O. cf. ovata, O. cf. siamensis, one non-PLTX producing species, O. rhodesae and compared them to a close phylogenetic relative and non-PLTX producer, Coolia malayensis. We found no clear differences in the presence or diversity of ketosynthase and ketoreductase transcripts between PLTX producing and non-producing Ostreopsis and Coolia species, as both groups contained >90 and > 10 phylogenetically diverse ketosynthase and ketoreductase transcripts, respectively. We report for the first-time type I single-, multi-domain polyketide synthases (PKSs) and hybrid non-ribosomal peptide synthase/PKS transcripts from all species. The long multi-modular PKSs were insufficient by themselves to synthesize the large complex polyether backbone of PLTX-like compounds. This implies that numerous PKS domains, including both single and multi-, work together on the biosynthesis of PLTX-like and other related polyketide compounds.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT120100704
dc.relation.ispartof	Environmental Microbiology	en_US
dc.relation.isbasedon	10.1111/1462-2920.14780	en_US
dc.subject.classification	Microbiology	en_US
dc.title	Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	21	en_US
utslib.for	0603 Evolutionary Biology	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	21	en_US

Abstract:

© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd. In marine ecosystems, dinoflagellates can become highly abundant and even dominant at times, despite their comparatively slow growth. Their ecological success may be related to their production of complex toxic polyketide compounds. Ostreopsis species produce potent palytoxin-like compounds (PLTX), which are associated with human skin and eye irritations, and illnesses through the consumption of contaminated seafood. To investigate the genetic basis of PLTX-like compounds, we sequenced and annotated transcriptomes from two PLTX-producing Ostreopsis species; O. cf. ovata, O. cf. siamensis, one non-PLTX producing species, O. rhodesae and compared them to a close phylogenetic relative and non-PLTX producer, Coolia malayensis. We found no clear differences in the presence or diversity of ketosynthase and ketoreductase transcripts between PLTX producing and non-producing Ostreopsis and Coolia species, as both groups contained >90 and > 10 phylogenetically diverse ketosynthase and ketoreductase transcripts, respectively. We report for the first-time type I single-, multi-domain polyketide synthases (PKSs) and hybrid non-ribosomal peptide synthase/PKS transcripts from all species. The long multi-modular PKSs were insufficient by themselves to synthesize the large complex polyether backbone of PLTX-like compounds. This implies that numerous PKS domains, including both single and multi-, work together on the biosynthesis of PLTX-like and other related polyketide compounds.

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http://hdl.handle.net/10453/139046