Surface plasmon enhanced light scattering biosensing: Size dependence on the gold nanoparticle tag

Yang, CT; Xu, Y; Pourhassan-Moghaddam, M; Tran, DP; Wu, L; Zhou, X; Thierry, B

Surface plasmon enhanced light scattering biosensing: Size dependence on the gold nanoparticle tag

Yang, CT Xu, Y Pourhassan-Moghaddam, M Tran, DP Wu, L Zhou, X Thierry, B

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Publication Type:: Journal Article
Citation:: Sensors (Switzerland), 2019, 19 (2)
Issue Date:: 2019-01-02

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Field	Value	Language
dc.contributor.author	Yang, CT	en_US
dc.contributor.author	Xu, Y	en_US
dc.contributor.author	Pourhassan-Moghaddam, M	en_US
dc.contributor.author	Tran, DP	en_US
dc.contributor.author	Wu, L	en_US
dc.contributor.author	Zhou, X	en_US
dc.contributor.author	Thierry, B	en_US
dc.date.accessioned	2020-03-17T03:38:59Z
dc.date.available	2019-01-11	en_US
dc.date.available	2020-03-17T03:38:59Z
dc.date.issued	2019-01-02	en_US
dc.identifier.citation	Sensors (Switzerland), 2019, 19 (2)	en_US
dc.identifier.issn	1424-8220	en_US
dc.identifier.uri	http://hdl.handle.net/10453/139360
dc.description.abstract	© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Surface plasmon enhanced light scattering (SP-LS) is a powerful new sensing SPR modality that yields excellent sensitivity in sandwich immunoassay using spherical gold nanoparticle (AuNP) tags. Towards further improving the performance of SP-LS, we systematically investigated the AuNP size effect. Simulation results indicated an AuNP size-dependent scattered power, and predicted the optimized AuNPs sizes (i.e., 100 and 130 nm) that afford extremely high signal enhancement in SP-LS. The maximum scattered power from a 130 nm AuNP is about 1700-fold higher than that obtained from a 17 nm AuNP. Experimentally, a bio-conjugation protocol was developed by coating the AuNPs with mixture of low and high molecular weight PEG molecules. Optimal IgG antibody bioconjugation conditions were identified using physicochemical characterization and a model dot-blot assay. Aggregation prevented the use of the larger AuNPs in SP-LS experiments. As predicted by simulation, AuNPs with diameters of 50 and 64 nm yielded significantly higher SP-LS signal enhancement in comparison to the smaller particles. Finally, we demonstrated the feasibility of a two-step SP-LS protocol based on a gold enhancement step, aimed at enlarging 36 nm AuNPs tags. This study provides a blue-print for the further development of SP-LS biosensing and its translation in the bioanalytical field.	en_US
dc.relation.ispartof	Sensors (Switzerland)	en_US
dc.relation.isbasedon	10.3390/s19020323	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Gold	en_US
dc.subject.mesh	Immunoglobulin G	en_US
dc.subject.mesh	Immunoconjugates	en_US
dc.subject.mesh	Immunoassay	en_US
dc.subject.mesh	Surface Plasmon Resonance	en_US
dc.subject.mesh	Biosensing Techniques	en_US
dc.subject.mesh	Metal Nanoparticles	en_US
dc.subject.mesh	Dynamic Light Scattering	en_US
dc.title	Surface plasmon enhanced light scattering biosensing: Size dependence on the gold nanoparticle tag	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	19	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0805 Distributed Computing	en_US
utslib.for	0906 Electrical and Electronic Engineering	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0602 Ecology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	19	en_US

Abstract:

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Surface plasmon enhanced light scattering (SP-LS) is a powerful new sensing SPR modality that yields excellent sensitivity in sandwich immunoassay using spherical gold nanoparticle (AuNP) tags. Towards further improving the performance of SP-LS, we systematically investigated the AuNP size effect. Simulation results indicated an AuNP size-dependent scattered power, and predicted the optimized AuNPs sizes (i.e., 100 and 130 nm) that afford extremely high signal enhancement in SP-LS. The maximum scattered power from a 130 nm AuNP is about 1700-fold higher than that obtained from a 17 nm AuNP. Experimentally, a bio-conjugation protocol was developed by coating the AuNPs with mixture of low and high molecular weight PEG molecules. Optimal IgG antibody bioconjugation conditions were identified using physicochemical characterization and a model dot-blot assay. Aggregation prevented the use of the larger AuNPs in SP-LS experiments. As predicted by simulation, AuNPs with diameters of 50 and 64 nm yielded significantly higher SP-LS signal enhancement in comparison to the smaller particles. Finally, we demonstrated the feasibility of a two-step SP-LS protocol based on a gold enhancement step, aimed at enlarging 36 nm AuNPs tags. This study provides a blue-print for the further development of SP-LS biosensing and its translation in the bioanalytical field.

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http://hdl.handle.net/10453/139360